Molecular and conventional methods for detection of Campylobacter spp. in retail poultry cuts, offal and chicken products | ||
| Benha Veterinary Medical Journal | ||
| Article 8, Volume 36, Issue 1, March 2019, Pages 71-76 PDF (793.06 K) | ||
| DOI: 10.21608/bvmj.2019.83228 | ||
| Authors | ||
| Amani Salem1; Nahla Abou El Roos2; Yahya Nassar2 | ||
| 1Food Control Dept., Fac. Vet. Med., Benha Univ. | ||
| 2Animal Health Research Institute, Shebin El koom Branch | ||
| Abstract | ||
| The goal of this study is to determine the prevalence of Campylobacter spp. in some poultry products by using both conventional and molecular methods. Two hundred chicken product samples were collected from various supermarkets at Menofia government (25g of each) of chicken breast with skin & without skin, thigh with skin &without skin, liver, gizzard, nuggets and luncheon. Most samples were contaminatedwith Campylobacter spp. except chicken luncheon. Chicken liver showed the highest contamination (92%) followed by gizzard(80%), thigh with skin (72%), thigh without skin (68%), breast with skin (60%) and breast without skin (48%). The lowest contamination was Chicken nuggets of 20 %. Campylobacter.jejuni (33.1%) was the most isolated serotype, and also followed by C.coli(16.6%), C.lari (6.9%), C.cinaedi(1.7%) , C.butzieri (1.7%) and C.upsaliens(1.1%) . hipO gene was detected in isolated C. jejuni.Virulence genes cdtA, cdtB and cdt C , cdtA and cdtC ,cdtB and cdtC ,CdtA, CdtB and CdtC were 30% , 15%,25%, 10% ,15 and 5 of isolated strains of C.jejuni, respectively. Finally the application of molecular methods is more favorable than conventional methods in detection of Campylobacter serotypes. | ||
| Keywords | ||
| Chicken meat; Campylobacter jejuni; liver; nuggets; PCR | ||
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