Attia, A., EL-Emery, M., Fayed, E. (2012). VERIFICATION OF VARIETALS PURITY IN MAIZE SEEDS USING RAPD-PCR TECHNIQUE. test, 3(4), 627-638. doi: 10.21608/jpp.2012.84203
A. N. Attia; M. I. EL-Emery; Eman A. Fayed. "VERIFICATION OF VARIETALS PURITY IN MAIZE SEEDS USING RAPD-PCR TECHNIQUE". test, 3, 4, 2012, 627-638. doi: 10.21608/jpp.2012.84203
Attia, A., EL-Emery, M., Fayed, E. (2012). 'VERIFICATION OF VARIETALS PURITY IN MAIZE SEEDS USING RAPD-PCR TECHNIQUE', test, 3(4), pp. 627-638. doi: 10.21608/jpp.2012.84203
Attia, A., EL-Emery, M., Fayed, E. VERIFICATION OF VARIETALS PURITY IN MAIZE SEEDS USING RAPD-PCR TECHNIQUE. test, 2012; 3(4): 627-638. doi: 10.21608/jpp.2012.84203

VERIFICATION OF VARIETALS PURITY IN MAIZE SEEDS USING RAPD-PCR TECHNIQUE

Journal of Plant Production
Article 3, Volume 3, Issue 4, April 2012, Pages 627-638    PDF (474.57 K)
Document Type: Original Article
DOI: 10.21608/jpp.2012.84203
Authors
A. N. Attia1; M. I. EL-Emery2; Eman A. Fayed2
1Agronomy Dept., Fac. Agric., Mans. Univ., Egypt
2Seed Technology Res. Dept., Agric. Res. Center, Egypt
Abstract
Laboratory experiments Randomly Amplified Polymorphic DNA (RAPD-PCR) was carried out during the 2010 season. RAPD-PCR products from five maize genotypes generated by random primer A03 with molecular weight of (1327, 1033, 904, 727, 595, 538, 487, 412, 360, 331, 280, 245 and 200 b.p.), primer B05 with molecular weight of (1327, 1033, 904, 727, 595, 538 and 487), primer B09 with molecular weight of (1948, 1513, 1233, 1020, 844, 578, 435, 354, 293 and 243 b.p.) primer B11 with molecular weight of (1232,  1038, 917, 726, 651, 574, 507, 455, 395, 328, 303, 263 and 218 b.p.) and primer B16 with molecular weight of (1554, 1203, 1001, 865, 657 and 518 b.p.). DNA pattern of genotypes using primers A03, B05, B09, B11 and B16 produced 13, 7, 10, 13 and 6 bands, respectively. RAPD-PCR products from the five maize genotypes generated by the random primers as visualized on 1% Agarose gel electrophoresis stained with 10 mg/ml ethidium bromide. The DNA was extracted from maize leaves tissue using Dellaporta extraction. (M)100 bp Marker, lanes from 1-15 is different five Maize genotypes.
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