Toxicity of Thymol on the Ultra-Scanning Structure of Skin and Digestive Gland Proteins of the Two Slugs ‘Limax maximus and Lehmannia marginata’ | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 23, Volume 71, Issue 6, April 2018, Pages 3405-3415 PDF (1.84 M) | ||
| Document Type: Original Article | ||
| Author | ||
| Omaima M. Mustafa | ||
| Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Egypt | ||
| Abstract | ||
| Background: thymol is a natural derivative of herb thyme and extracted from Thymus vulgaris. Aim of the work: the present investigation was carried out to study the side effects of thymol on the skin and protein content in the digestive gland of both slugs: Limax maximus and Lehmannia marginata. Materials and Methods: the slugs are classified into three groups: the first one served as a control, the second and third groups fed on LC50 and LC90 of thymol for 48 hours. Results: the histological and scanning electron microscopic (SEM) observations of skin of LC90-treated slugs exhibited erosion of the epithelial cells, hypoplasia of the connective tissues with increased mucus secretion. Moreover, the different protein bands of the control and treated slugs with LC50 and LC90 of thymol were demonstrated by SDS-PAGE technique. A total number of 37 different protein bands were ranged from 5.181 to 84.375 kDa. Conclusion: the present study supported the use of thymol as a molluscicide agent on the skin and digestive gland proteins of both slugs (Limax maximus and Lehmannia marginata) that offers a safe alternative to other more persistent chemical pesticides that can be dispersed in runoff and produce subsequent contamination. | ||
| Keywords | ||
| Digestive gland; proteins; skin; SEM; Slugs; Thymol | ||
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