ANTHER CULTURE AND PLANT REGENERATION IN CITRUS (Citrus volkameriana ). | ||
| Journal of Plant Production | ||
| Article 10, Volume 2, Issue 12, December 2011, Pages 1717-1729 PDF (467.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2011.85773 | ||
| Author | ||
| H. M. S. Aboshama* | ||
| Minufiya University, Genetic Engineering and Biotechnology Institute, Plant Biotechnology Department, | ||
| Abstract | ||
| Development of an efficient and cost-effective doubled haploid production system in Citrus volkameriana is the prerequisite for the application of doubled haploid technology in a practical breeding program. An improvement of the protocol for haploid induction through anther culture of Citrus volkameriana was achieved following the evaluation of a number of the factors affecting androgenesis. Two types of media were tested for response of anther culture. In induction cultures, N6 Chu (1978) basal medium was more effective than Murashige and Skoog (1962). The influence of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) and three temperature pre-treatments (4oC, 25oC, 32oC) on the floral buds with respect to anther culture of C. volkameriana . As well as, the effect of AgNO3 concentration on Volkamer” lemon embryogenesis was investigated. The highest percentages of anthers producing embryoids (47.50) was produced on N6 medium at the concentration of 2.0 mg/l 2,4-D and 0.5 mg/l BA together. Pre-treatment of temperatures at 4oC and 25oC were more favorable for embryo production than 32oC. Silver nitrate concentrations had a strong influence on embryo yields where a 2.0 mg /l significantly gave a higher percentages of anthers (65.00%) producing embryoids compared with 1.0 mg /l or 0.0 mg/l (55.00, 45.83%, respectively). Regeneration of androgenic haploid plantlets from C. volkameriana is reported in this study. As embryogenesis proceeded, the normal pattern of development was observed, including heart-shape, torpedo and cotyledonar embryos. | ||
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