Sayed, R., Abdalla, R., El Sayed, T., Rizk., S. (2020). Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation. test, 13(2), 129-140. doi: 10.21608/eajbsa.2020.85798
Rehab M Sayed; R. S. Abdalla; T. S. El Sayed; S. A. Rizk.. "Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation". test, 13, 2, 2020, 129-140. doi: 10.21608/eajbsa.2020.85798
Sayed, R., Abdalla, R., El Sayed, T., Rizk., S. (2020). 'Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation', test, 13(2), pp. 129-140. doi: 10.21608/eajbsa.2020.85798
Sayed, R., Abdalla, R., El Sayed, T., Rizk., S. Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation. test, 2020; 13(2): 129-140. doi: 10.21608/eajbsa.2020.85798

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 9, Volume 13, Issue 2, June 2020, Pages 129-140    PDF (652.23 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2020.85798
Authors
Rehab M Sayed1; R. S. Abdalla2; T. S. El Sayed3; S. A. Rizk.3
1Natural Products Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority
2Natural Products Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
3Natural Products Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Abstract
Radiation technology is widely used to produce changes in biosystems. The goal of this work is to determine the variation induced in male parents and F1 Ceratitiis capitata when gamma-irradiated as pupae using substerilising doses (50 and 70 Gy and sterilising dose 90 Gy. Biological studies showed that the percent adult emergence of irradiated pupae decreased as the dose increased and also the percent egg hatchability decreased as the dose increased, also percent pupation and percent emergence of F1 decreased as the dose increased. Comparing DNA (using RAPD-PCR) of untreated adults, adults irradiated with sterilising dose of 90 Gy and parents irradiated with substerile dose (50 and 70 Gy) and their resultant F1. The results revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that irradiation of parents with 50 and 70 Gy and their F1 are different from the unirradiated pupae It was concluded that the sterile male technique could be used as a benefit tool in controlling Ceratitiis capitata. 
Keywords
Mediterranean fruit fly; gamma radiation; substerile; DNA; RAPD-PCR; bands
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