Helal, A., Helal, O., Metwaly, H., Abo Gazia, M., Mohamed, O. (2020). Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat. test, 37(1), 193-206. doi: 10.21608/bmfj.2020.85984
Azza Ibrahim Helal; Omayma Helal; Hala Metwaly; Maha Abo Gazia; Ola Mohamed. "Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat". test, 37, 1, 2020, 193-206. doi: 10.21608/bmfj.2020.85984
Helal, A., Helal, O., Metwaly, H., Abo Gazia, M., Mohamed, O. (2020). 'Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat', test, 37(1), pp. 193-206. doi: 10.21608/bmfj.2020.85984
Helal, A., Helal, O., Metwaly, H., Abo Gazia, M., Mohamed, O. Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat. test, 2020; 37(1): 193-206. doi: 10.21608/bmfj.2020.85984

Histological and immunohistochemical study on the possible therapeutic role of stem cells and curcumin in cyclophosphamide-induced cardiotoxicity in adult male albino rat

Benha Medical Journal
Article 5, Volume 37, Issue 1, May 2020, Pages 193-206    PDF (1.59 M)
Document Type: Original Article
DOI: 10.21608/bmfj.2020.85984
Authors
Azza Ibrahim Helal* 1; Omayma Helal2; Hala Metwaly3; Maha Abo Gazia4; Ola Mohamed2
1Histology department,faculty of medicine,kafr sheikh university
2Department of histology and cell biology, Benha faculty of medicine
3department of clinical pathology, Cairo university
4department of Histology, Benha faculty of medicine, kafr sheikh university
Abstract
Introduction: Cyclophosphamide (CP) is used as a chemotherapeutic and an immunosuppressive agent. CP is known to cause multiple organ toxicity; the most obvious is cardiotoxicity. Aim: Possibile  therapeutic effect on bone marrow -derived mesenchymal   stem cells (BM-MSCs) in ameliorating CP- induced cardiotoxicity compared with Curcumin in albino rats. Methods: Fifty male albino rats were divided into five groups: group I received intraperitoneal injection of sterile water; group II received oral curcumin (200 mg/kg); group III received only one intraperitoneal injection of CP (200 mg/kg) and were sacrificed on day 40; group IV  received the same treatment as group III  followed by oral administration of curcumin from day 10 and were sacrificed on day 40; group V received the same treatment as group III followed by a single BM- MSCs injection intraperitoneally at a dose of 1×106 cells/ rat on day 10 and were sacrificed on day 40. Results: Histological structure of the cardiac muscle by light and electron microscopic examination revealed marked structural changes in rats treated with CP alone. Improvement in BM-MSCs group more than curcumin treated group was observed. Immunohistochemical staining of the cardiac muscle showed strong positive immunoreactivity for caspase-3 in group III compared to the control and other groups. Also, BM-MSCs extensively reduced the amount of collagen fibers compared with other groups. Conclusion: The use of curcumin has a limited beneficial effect on the protection of cardiac muscle against CP toxicity compared with stem cells.
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