Muhanna, N., Elwan, S., Dib, N. (2018). Biological Control of Root Rot Complex of Pea (Pisum sativum L.). test, 46(1), 49-67. doi: 10.21608/ejp.2018.87766
Naglaa A.S. Muhanna; Safa E. Elwan; Nsreen D. Dib. "Biological Control of Root Rot Complex of Pea (Pisum sativum L.)". test, 46, 1, 2018, 49-67. doi: 10.21608/ejp.2018.87766
Muhanna, N., Elwan, S., Dib, N. (2018). 'Biological Control of Root Rot Complex of Pea (Pisum sativum L.)', test, 46(1), pp. 49-67. doi: 10.21608/ejp.2018.87766
Muhanna, N., Elwan, S., Dib, N. Biological Control of Root Rot Complex of Pea (Pisum sativum L.). test, 2018; 46(1): 49-67. doi: 10.21608/ejp.2018.87766

Biological Control of Root Rot Complex of Pea (Pisum sativum L.)

Egyptian Journal of Phytopathology
Article 4, Volume 46, Issue 1, June 2018, Pages 49-67    PDF (295.9 K)
Document Type: Original Article
DOI: 10.21608/ejp.2018.87766
Authors
Naglaa A.S. Muhanna* 1; Safa E. Elwan1; Nsreen D. Dib2
1Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt.
2Agric. Sci. Res. Center, Lattakia, Syria
Abstract
Pea root rot complex, caused by several soil borne fungi is a global major yield-limiting factor for pea production. Fusarium oxysporum, Rhizoctonia solani Kühn, Sclerotinia sclerotiorum, and Thielaviopsis basicola were isolated, identified and confirmed in pathogenicity test as root rot pathogens involved. Thin layer chromatography (TLC) technique revealed that the filtrates of R. solani, F. oxysporum, S. sclerotiorum and T. basicola contained a variety of toxins that were collected from the chromatogram bands. Fusarium oxysporum (one fraction) produced fusaric acid. Sclerotinia
sclerotiorum (two fractions) produced Sclerotinin A. Control of root rot complex of pea using biological agents (BCAs) as Trichoderma harzianum, Rhizobium sp < /em> and Bacillus subtilis was studied to elucidate their disease control efficacy. Soil treated with B. subtilis, T. harzianum, Rhizobium sp < /em> significantly decreased the disease severity after treatment. The treatments gave the highest valued of plant growth parameters. Results showed that the enzyme activities of PG
and Cx decreased in treated root pea plants compared to untreated ones such enzymes are supposedly involved in pathogenesis. Further studies are needed to define the best application methods to achieve the most effective disease control.
Keywords
Pea; Pisum sativum; Bio-agents; disease complex; enzyme; Root rot; toxins; Wilt; Fusarium oxysporum; Rhizoctonia solani; Sclerotinia sclerotiorum; Thielaviopsis basicola
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