IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 3, Volume 46, Issue 2, August 2016, Pages 253-260 PDF (3.94 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2016.88648 | ||
| Authors | ||
| NEVEEN A. MADBOULY1; ALYA FARID1; AYMAN A. EL-BADRY2; AZZA M. EL-AMIR1 | ||
| 1Department of Zoology, Faculty of Science, Cairo University, Giza 12613, Egypt. | ||
| 2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| Abstract | ||
| Giardiasis is a major health problem in both developed and developing world. A variety of methods for diagnosis of Giardia duodenalis cysts or trophozoites is available but still has certain limitations. 100 sample from diarrhoeal children who attending outpatient clinic in Abu El Rish hospital, Kasr Al Ainy, Faculty of Medicine, Cairo University, Egypt. Giardiasis was diagnosed by direct wet mount, microscopy after formal- ethyl acetate concentration, Ridascreen ELISA assay and n-PCR targeting beta giardin (bg) gene. Using ELISA as reference standard, the methods' sensitivities, specificities, positive (PPV) and negative (NPV) predictive values and positive (LR+) and negative (LR-) likelihood ratios with 95% confidence interval (95% CI) were analyzed.The diagnostic methods were evaluated to determine their impact on the posttest probability using Fagan’s nomogram. All the studied methods led to a LR+ higher than 10 indicating ability to ruling in giardiasis. n-PCR recorded LR- equal 0.00 and the probability of giardiasis would be 0% if the test was negative. The methods were also ranked on basis of Multiattribute utility theory and Analytical hierarchy process with ELISA ranked better than n-PCR. | ||
| Keywords | ||
| Giardia; Copro-DNA; bg gene; Ridascreen ELISA; Fagan's nomogram | ||
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