GHETAS, A., KUTKAT, M., AMER, M., AWAAD, M. (2016). ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT. test, 46(2), 341-346. doi: 10.21608/jesp.2016.88682
ALY M. GHETAS; MOHAMMAD A. KUTKAT; MOHAMMAD M. AMER; MOHAMMAD H. H. AWAAD. "ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT". test, 46, 2, 2016, 341-346. doi: 10.21608/jesp.2016.88682
GHETAS, A., KUTKAT, M., AMER, M., AWAAD, M. (2016). 'ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT', test, 46(2), pp. 341-346. doi: 10.21608/jesp.2016.88682
GHETAS, A., KUTKAT, M., AMER, M., AWAAD, M. ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT. test, 2016; 46(2): 341-346. doi: 10.21608/jesp.2016.88682

ISOLATION AND MOLECULAR IDENTIFICATION OF IBV ISOLATES IN DIFFERENT GOVERNORATES IN EGYPT

Journal of the Egyptian Society of Parasitology
Article 9, Volume 46, Issue 2, August 2016, Pages 341-346    PDF (2.47 M)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88682
Authors
ALY M. GHETAS1; MOHAMMAD A. KUTKAT1; MOHAMMAD M. AMER2; MOHAMMAD H. H. AWAAD2
1Departments of Poultry Diseases, National Research Centre, Egypt.
2Departments of Poultry Diseases, National Research Centre, and Faculty of Veterinary Medicine, Cairo University, Egypt.
Abstract
Tracheal swabs and different organs are collected from 17 chicken farms showing respiratory signs and variable mortalities in different governorates. Three successive blind serial passages were performed. Four IBV isolates are detected in vaccinated chickens by RT-PCR and are identified by sequence and phylogenetic analysis of portion of S1 gene. Two IBV isolates, IBV S40 and IBV S61, are related to Mass reference strains (Egypt/F/03, M41, H120, Ma5, and M52). However, IBV S78 and IBV S82 are related to Egyptian variant 2 IBV strains Ck/Eg/BSU- 2/2011 and Ck/Eg/ BSU-3/2011.These results indicate the continuous evolution of Egyptian IBV circulating in chickens despite vaccination using H120 live attenuated vaccine.
Keywords
IBV; isolation; identification; vaccine; Egypt
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