EVALUATION OF COMMON MICROSCOPIC TECHNIQUES FOR DETECTION OF BLASTOCYSTIS HOMINIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 4, Volume 50, Issue 1, April 2020, Pages 33-40 PDF (796.48 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2020.88748 | ||
| Authors | ||
| WAAD T. ALDAHHASI1; FAWZIA H. TOULAH1; MAJED H. WAKID2, 3 | ||
| 1Department of Biology, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia. | ||
| 2Department of Medical Laboratory Technology, Special Infectious Agent Unit, King Fahd .+ | ||
| 3Medical Research Center3, Faculty of Applied Medical Sciences, P. O. Box 80324, King Abdul Aziz University, Jeddah 21589,, Saudi Arabia. | ||
| Abstract | ||
| Blastocystis hominis is the most prevalent eukaryotic intestinal protozoa that colonize the human gut, associated with intestinal disorders, with pathogenic controversial as most of them are asymptomatic. In this study, a comparison was carried out for sensitivity and specificity among two common using light microscopy, direct wet smear and Ritchie for the diagnosis B. hominis in human stools. Stool samples were collected from 29 Saudi and 111 non-Saudi patients, and examined for the detection of B. hominis. The infection was among age group between 31-50 years old and the highest rate was among male patients. Also, the results revealed that 98 samples were positive by direct smears; the sensitivity and specificity were 100% &75.9% respectively. Meanwhile, Ritchie technique revealed 86 positive samples, the sensitivity and specificity were 99% and 90.9% respectively. | ||
| Keywords | ||
| Saudi Arabia; Blastocystis hominis; parasites; direct smears; Ritchie | ||
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