IN VIVO AND IN VITRO EFFICACY OF NIGELLA SATIVA AQUEOUS EXTRACT ON BLASTOCYSTIS HOMINIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 4, Volume 46, Issue 1, April 2016, Pages 27-34 PDF (3.91 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2016.88928 | ||
| Authors | ||
| OMIMA M. EIDA1, 2; HANAA A. EL-SHAFEI3; YOUSRA A. NOMEIR4; MOHAMMED B. EL SAFHI4 | ||
| 1Departments of Pharmaceutics, Faculty of Pharmacy, Jazan University, P.O. Box 114, Postal Code 45 142 Saudi Arabia.+ | ||
| 2Department of Parasitology, Faculty of Medicine, Suez Canal University, Egypt | ||
| 3Departments of Pharmaceutics, Faculty of Pharmacy, Jazan University, P.O. Box 114, Postal Code 45 142 Saudi Arabia. | ||
| 4Departments of Pharmacology, Faculty of Pharmacy, Jazan University, P.O. Box 114, Postal Code 45 142 Saudi Arabia. | ||
| Abstract | ||
| Metronidazole (MTZ) was the most widely accepted treatment for Blastocystis hominis (B. hominis) with high treatment failure rate, resistance and potential mutagenic and carcinogenic effects so there is urgent need to find out new, effective and safe treatment against B. hominis. The present research aimed to evaluate the therapeutic effect of the aqueous extract of Nigella sativa (NS) at different doses on B. hominis in vitro and in vivo in comparison to MTZ as a control drug. Isolates of B. hominis were obtained from patients complaining of diarrhea and abdominal pain. Isolates were cultured in egg diphasic medium (LE) for in vitro study and to adjust proper inoculating dose for in vivo study. The aqueous extract of NS at concentrations of 100 & 500μg/ml showed a potent lethal effect on B. hominis isolates in vitro. Caecal tissue of experimentally infected and treated mice with two different doses of NS (250 & 500mg/kg/d) were examined histopathologically and compared with that of mice infected and treated by two doses of MTZ (62 & 125 mg/kg/d) as control drug and Infected untreated mice as negative control group. Histopathological examination of infected untreated group showed all pathological degrees in the caecal tissue while infected treated one showed remission of pathological changes especially with higher dose (500mg/kg). Present study proved that N. sativa had inhibitory effect on B. hominis in vitro and prevented cytopathic effect in infected mice inoculated orally with B. hominis. | ||
| Keywords | ||
| B. hominis; Nigella sativa; in vivo; in vitro | ||
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