Multiplex PCR Assay for Identification of Brucella Strains including the vaccinal Strains and its differentiation from Yersinia O: 9 | ||
| Benha Veterinary Medical Journal | ||
| Article 22, Volume 37, Issue 1, September 2019, Pages 107-111 PDF (822.13 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2019.15178.1055 | ||
| Authors | ||
| Rania Yahya Abo-Sakaya* 1; Ashraf Awad Abd El-Tawab2; Fatma Elhofy2; Amany Ibrahim Baghdadi3 | ||
| 1Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt | ||
| 2Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt | ||
| 3Researcher at Veterinary Serum and Vaccine Research Institute, Cairo, Egypt | ||
| Abstract | ||
| In this comparative study, all the five Brucella strains were morphologically, serologically and biochemically identified using media different stains and antibiotics. Multiplex PCR was used to differentiate between the Brucella strains and Yersinia enterocolitica O: 9. five primer sets were designed to the most specific variable regions of the different Brucella species. The results of multiplex PCR were very successful and accurate in terms of characterization and typing of the Brucella vaccine, reference and wild Brucella strains from Yersinia which give false positive while using tests based on anti-LPS antibodies but with PCR for DNA of Yersinia give negative. The molecular typing of the Brucella strains by multiplex PCR had several advantages over the use of the conventional methods being very fast, precise, easier, more sensitive, and economic and could be applied on minimal sample preparation. The development of this PCR method is the first step toward the development of a novel kit for the molecular identification of Brucella strains from other Gram- negative bacteria. | ||
| Keywords | ||
| Brucella Typing; Multiplex PCR; Yersinia enterocolitica; molecular identification | ||
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