Abd El Tawab, A., Mohamed, A., Ammar, A., Mohamed, M. (2019). Isolation and identification of Cronobacter species from some animal sources. test, 37(1), 112-117. doi: 10.21608/bvmj.2019.17855.1106
Ashraf Abd El Tawab; Amira Mohamed; Ahmed Ammar; Mohamed Mohamed. "Isolation and identification of Cronobacter species from some animal sources". test, 37, 1, 2019, 112-117. doi: 10.21608/bvmj.2019.17855.1106
Abd El Tawab, A., Mohamed, A., Ammar, A., Mohamed, M. (2019). 'Isolation and identification of Cronobacter species from some animal sources', test, 37(1), pp. 112-117. doi: 10.21608/bvmj.2019.17855.1106
Abd El Tawab, A., Mohamed, A., Ammar, A., Mohamed, M. Isolation and identification of Cronobacter species from some animal sources. test, 2019; 37(1): 112-117. doi: 10.21608/bvmj.2019.17855.1106

Isolation and identification of Cronobacter species from some animal sources

Benha Veterinary Medical Journal
Article 23, Volume 37, Issue 1, September 2019, Pages 112-117    PDF (827.77 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.17855.1106
Authors
Ashraf Abd El Tawab1; Amira Mohamed* 2; Ahmed Ammar3; Mohamed Mohamed4
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
2Faculty of Veterinary Medicine, Benha Univ., Egypt
3Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University
4Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University
Abstract
This work was carried out to study the bacteriological importance of Cronobacter sakazakii as a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates due to consumption of contaminated infant powdered infant formula (milk and food). A total of 100 samples [PIF milk (n=55), PIF food (n=15), milk powder (n=15) and milk powder products (n=15) were collected and subjected to bacteriological examination for the presence of Cronobacter. Six samples out of 100 examined (6%) were found positive for Cronobacter spp. The isolation rates were 4% in PIF milk and 1% in PIF food. The identity of the isolated organism was confirmed as Cronobacter spp. by subjecting the bacteriologically positive samples to PCR technique using 16S rRNA species specific primers. Cronobacter specific 16S rRNA was detected respectively in 3/5 and 1/5 of bacteriologically positive PIF milk and PIF food examined. All positive 16S rRNA (n=4) were examined for the presence of C. sakazakii. C. sakazakii were confirmed in an isolate from 3 isolates of PIF milk and one of PIF food. The outer membrane protein A (ompA) gene was detected in 2 identified C. sakazakii isolates, while gene encoding for zinc-metaloprotease (zpx) was only identified in 3 C. sakazakii isolates
Keywords
Cronobacter; animal; Bacteria; Milk powder
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