Review Article: Molecular Diagnosis of Human Papilloma Virus Infection | ||
| The Medical Journal of Cairo University | ||
| Article 60, Volume 88, March, March 2020, Pages 471-480 PDF (190.16 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjcu.2020.94013 | ||
| Authors | ||
| AHMED ABOULNASR, M.D.; NADINE SHERIF, M.D.; MOHAMED ALI, M.Sc.; MOHAMED ELMAHY, M.D.; MOHAMED SHALABY, M.D.; AMAL SOLIMAN, M.D.; MOSTAFA SALEM, M.D. | ||
| The Departments of Obstetrics & Gynecology*, Clinical Pathology** and Pathology***, Faculty of Medicine, Cairo University | ||
| Abstract | ||
| Abstract Infection with human papillomaviruses (HPVs) can cause warts on cutaneous epithelium, while in the anogenital region these viruses can cause both genital warts and various forms of cancer in men and women. The main interest in HPV relates to its causative role in cervical cancer. Most HPV infections in young women resolve spontaneously, most frequently within a 24-month period. Identification of HPV genotypes would require the use of type-specific probes in multiple in situ hybridization experiments. Alternatively, HPV-DNA can be directly isolated from clinical samples and detected by Southern blot or dot spot hybridization. However, such approaches are insensitive, labor intensive and unsuitable for high through put screening. Therefore, nucleic acid amplification methods have been developed to increase the sensitivity as well as the specificity of HPV-DNA detection. | ||
| Keywords | ||
| Human papilloma virus; Infection; PCR | ||
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