Jansi, P., Padua, J., Freeda, R. (2020). In Vivo Toxic and Teratogenic Effects of Biologically Synthesized Silver Nanoparticles in The Embryos of the Zebrafish, Danio rerio.. test, 12(1), 81-92. doi: 10.21608/eajbsz.2020.94075
P.C. Jeba Preethi Jansi; Jeni Chandar Padua; R. Regis Freeda. "In Vivo Toxic and Teratogenic Effects of Biologically Synthesized Silver Nanoparticles in The Embryos of the Zebrafish, Danio rerio.". test, 12, 1, 2020, 81-92. doi: 10.21608/eajbsz.2020.94075
Jansi, P., Padua, J., Freeda, R. (2020). 'In Vivo Toxic and Teratogenic Effects of Biologically Synthesized Silver Nanoparticles in The Embryos of the Zebrafish, Danio rerio.', test, 12(1), pp. 81-92. doi: 10.21608/eajbsz.2020.94075
Jansi, P., Padua, J., Freeda, R. In Vivo Toxic and Teratogenic Effects of Biologically Synthesized Silver Nanoparticles in The Embryos of the Zebrafish, Danio rerio.. test, 2020; 12(1): 81-92. doi: 10.21608/eajbsz.2020.94075

In Vivo Toxic and Teratogenic Effects of Biologically Synthesized Silver Nanoparticles in The Embryos of the Zebrafish, Danio rerio.

Egyptian Academic Journal of Biological Sciences, B. Zoology
Article 7, Volume 12, Issue 1, June 2020, Pages 81-92    PDF (731.96 K)
Document Type: Original Article
DOI: 10.21608/eajbsz.2020.94075
Authors
P.C. Jeba Preethi Jansi1; Jeni Chandar Padua2; R. Regis Freeda2
1Department of Zoology, Holy Cross College (Autonomous), Nagercoil, Tamilnadu, India
2Department of Zoology, Holy Cross College (Autonomous), Nagercoil, TN, India
Abstract
In the current study, AgNPs synthesized using Bacopa monnieri were characterized by FTIR, UV-Spectrophotometry, XRD, and SEM measurements and subjected to diverse antimicrobial and toxicity assays. FTIR spectrum confirmed the involvement of amines, phenols, alkenes, amides, aromatic, and nitro compounds in the capping and reduction of AgNPs while XRD characterized the crystalline particles presenting a spherical structure around 24nm. SEM measurements showed a spherical and polygonal structure with size ranging from 35-45 nm indicating polydispersity. Comparative toxicity assays of Bacopa monnieri leaf extracts with nanosynthesized AgNPs on Danio rerio embryos, confirmed that mortality, hatchability, heartbeat rate, teratogenicity, and embryogenicity were concentration-dependent. Lethal effects were pronounced in nanosynthesised AgNPs whereas sub lethal and teratogenic toxicity effects like lordosis, scoliosis, edema and growth retardation were expressed in 84% of the exposed models. The incidence of scoliosis was found to be higher in all concentrations suggesting a possible alteration in the WNT Genes. The antimicrobial study confirmed the toxic effect of AgNPs of B. monnieri. This study gives newer dimensions in the furtherance of toxicity studies on green synthesized nanoparticles. 
Keywords
Silver Nanoparticles; Teratogenic Effects
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