DETECTION OF HELICOBACTER ANTIGEN IN STOOL SAMPLES AND ITS RELATION TO H. PYLORI POSITIVE CHOLECYSTITIS IN EGYPTIAN PATIENTS WITH CHRONIC CALCULAR CHOLECYSTITIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 13, Volume 45, Issue 3, December 2015, Pages 579-586 PDF (293.73 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2015.96301 | ||
| Authors | ||
| EHSAN H. HASSAN1; SHAWKAT S. GERGES2; REHAB AHMED3; ZEINAB M. MOSTAFA4; AZZA HASSAN5; HAGER ABD AL-HAMID5; HEBA ABD EL-GALIL6; SUZAN THABET1 | ||
| 1Department of Pathology, National Hepatology and Tropical Medicine, Research Institute, Cairo, Egypt. | ||
| 2Department of Surgery, National Hepatology and Tropical Medicine, Research Institute, Cairo, Egypt. | ||
| 3Department of General Medicine, National Hepatology and Tropical Medicine, Research Institute, Cairo, Egypt. | ||
| 4Department of Radiology, National Hepatology and Tropical Medicine, Research Institute, Cairo, Egypt. | ||
| 5Department of Medical Parasitology & Immunology, National Hepatology and Tropical Medicine, Research Institute, Cairo, Egypt. | ||
| 6Department of Community, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. | ||
| Abstract | ||
| Evidences supporting the association between H. pylori infection and chronic cholecystitis could be found by using direct culture or staining of H. pylori in gallbladder tissues as well as indirect techniques. Stool antigen test has been widely used due to its non-invasive nature. Various stool antigen tests were developed to detect H. pylori using an enzyme immunoassay (EIA) based on monoclonal or polyclonal antibodies This study evaluated the frequency of H. pylori antigen in stool samples of patients with chronic calcular cholecystitis as regard gall bladder histopathological changes. -Fifty patients were included presented with symptomatic cholecystolithiasis recruited from the outpatient clinic of National Hepatology and Tropical Medicine Research Institute during 2014-2015. Full history and clinical examination and abdominal ultrasonography were performed. Stool samples were collected, prepared and examined for detection of H. pylori antigen. Cholecystectomy was done for all patients; 45 patients (90%) by laparoscopic Cholecystectomy and 5 patients (10%) by open surgery and removed gallbladders were submitted to pathology department for detection of H. pylori in tissue under microscope using Giemsa stain. The results showed that (82%) were females with mean age (42.6+1years). The mean BMI was (29+7.2) H. pylori-specific antigen in stool samples was detected in 40% of patients and38% were detected in patients; tissue, with significant correlation between H. pylori-specific antigen in stool and in tissue. Histopathological pictures infection in tissue were 68.4% mucosal erosions, 63.2% mucosal atrophy, 57.9% mucosal hyperplasia, 26.3% metaplasia, 42.1% musculosa hypertrophy, 26.3% fibrosis, but lymphoid aggregates were in 42.1% of cases. | ||
| Keywords | ||
| Helicobacter pylori; chronic calcular cholecystitis; ELISA; histopathology | ||
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