DIRECT GENOTYPING OF TOXOPLASMA GONDII IN BLOOD SAMPLES FROM PREGNANT WOMEN IN JAZAN, SAUDI ARABIA | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 16, Volume 45, Issue 3, December 2015, Pages 603-608 PDF (231.97 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2015.96310 | ||
| Author | ||
| AMANY M. EIDA | ||
| Department of Parasitology, Faculty of Medicine, Suez Canal University, Egypt, and Jazan University, Saudi Arabia. | ||
| Abstract | ||
| Toxoplasma gondii (T. gondii) is divided three main clonal lineages designated as type I, II, and III and atypical genotypes were also detected. The distribution of T. gondii genotypes varied from one geographic area to another. This study characterized of T. gondii isolates from pregnant women in Jazan. Genetic analysis of the GRA6-coding fragment was performed for T. gondii genotyping using PCR-RFLP method. The seropositive for Toxoplasma-specific antibodies were determined using ELISA and were 27.9% in pregnant women in Saudi Arabia. Women seropositive for Toxoplasma IgG &IgM (GI=30) and for specific IgG (GII=30) were included. Among pregnant women, 83.3% of GI (women seropositive for IgG and IgM) and 90% of GII (women seropositive for IgG) were asymptomatic and observed clinical symptoms were fever (n=4) and cachexia (n=2) and lymphadenopathy (n=1). GRA6-nested PCR was positive in 8 blood samples (13.3%), 5 of GI & 3 of GII seropositive women. RFLP analysis showed the detection of genotype I in 8 samples with no cases coinciding to pattern of type II or type III. | ||
| Keywords | ||
| Saudi Arabia; Jazan; Pregnant women T. gondii; genotypes; GRA6; PCR-RFLP | ||
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