Influence Of Combinations Of Some Permeable Cryoprotectants With Chicken And Duck Egg Yolks On Freezability And Dna Integrity Of Buffalo Spermatozoa | ||
| Journal of Applied Veterinary Sciences | ||
| Article 6, Volume 5, Issue 3, July 2020, Pages 49-56 PDF (749.72 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2020.98371 | ||
| Authors | ||
| Rawash Z.M.1; Amal Z.A. Leil2; Abeer M Anwer3; El-Badry D.A.* 4 | ||
| 1Dept. of Artificial Insemination and Embryo Transfer Dept., Animal Reproduction Research Institute, Agricultural Research Center, Egypt | ||
| 2Field investigation Department, Animal Reproduction Research Institute, Giza, Egypt. | ||
| 3Immunology and Immunopharmacology Unit, Animal Reproduction Research Institute, Agriculture Research Center. Giza, Egypt | ||
| 4Dept. Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center. Giza, Egypt | ||
| Abstract | ||
| The present study aimed to investigate the cryoprotective effect of different combinations of glycerol (G), dimethyl sulphoxide (DMSO) and dimethylformamide (DMF) with chicken egg yolk (CEY) and duck egg yolk (DEY) on freezability of buffalo spermatozoa. Semen samples were collected from 4 buffalo bulls and diluted with Tris-based extender supplemented with 7% (V/V) cryoprotectant including G, DMSO, DMF or a mixture of 3.5% G + 3.5% DMSO, 3.5% G + 3.5% DMF and 3.5% DMSO + 3.5% DMF. All the fore-mentioned aliquots were divided into two equal portions; one is supplemented with chicken's egg yolk (CEY) and the other with duck's egg yolk (DEY). Post-thawing sperm motility, viability and acrosomal, plasmatic membrane and DNA integrities were assessed. The results clearly indicated that adding a mixture of 3.5% G + 3.5% DMSO with DEY to Tris extender significantly improved (p < 0.01) post-thawing sperm motility, viability index and percentage of spermatozoa with intact acrosomes, plasma membrane and DNA (58.33%±1.66%, 173.33±5.46, 69.00±1.58%, 65.00±1.15% and 96.91±0.13%, respectively) compared with the use of 7% G and CEY (51.50±1.44%, 146.25±6.49, 61.00±2.30, 58.00±1.15% and 91.40±0.17, respectively). In conclusion, motility and functional integrity of cryopreserved buffalo spermatozoa could be enhanced by using Tris extender containing a mixture of 3.5% glycerol + 3.5% DMSO with duck’s egg yolk. | ||
| Keywords | ||
| Buffalo; cryoprotectants; duck egg yolk; freezing; glycerol; spermatozoa | ||
| References | ||
|
AKHTER, S., RAKHA, B., ANSARI, M., HUSNA, A., IQBAL, S., AND KHALID, M. 2017. Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen. Theriogenology 87: 259–265.
ALI, ABT, BOMBOI, G., AND FLORIS, B. 2013. Replacing chicken yolk with yolks from other sources in ram semen diluents and their effects on fertility in vitro. Small Rum. Res. 113: 405–410.
AKHTER, S., ANSARI, MS, RAKHA, BA, ANDRABI, SMH, KHALID, M. AND ULLAH, N. 2011. Effect of low-density lipoproteins in extender on freezability and fertility of buffalo (Bubalus bubalis) bull semen. Theriogenology 76:759–764.
ALMADALY, EA, TAWFIK, FS, EL-KON, II, HELEIL, BA, AND FATTOUH, EM 2019. Effect of different cryoprotectants on the post-thaw sperm characteristics and in vivo fertility of buffalo (Bubalus bubalis) bull semen. Slov. Vet. Res. 56: 541–551
AMIRAT, L., TAINTURIER, D., JEANNEAU, L., THORIN, C., GÉRARD, O., COURTENS, JL, AND ANTON, M., 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology 61: 895–907.
ANDRABI, S., ANSARI, M., ULLAH, N., ANWAR, M., MEHMOOD, A., AND AKHTER, S. 2008. Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reprod. Sci. 104: 427–433.
ARIANTIE, OS, YUSUF, TL, SAJUTHI, D., AND ARIFIANTINI, RI, 2013. Effect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris Diluents. JITV. 18: 239-250.
BADR, MR, ABD EL-MALAK, MG, AND HASSAN, HM, 2010. Effect of trehalose on cryopreservation, oxidative stress and DNA integrity of buffalo spermatozoa. J Reprod. Infertil. 1:50-57.
BAREN, A., BACINOGLU, S., EVECEN, M., SAHIN, BE, ALKAN, S., DEMIR, K., AK, K., AND ILERI, K. 2004. Freezing of cat semen in straws with different GLY levels containing Tris-extender. Turk. J. Vet. Anim. Sci., 28: 545–552.
BATHGATE, R., MAXWELL, W., AND EVANS, G. 2006. Studies on the Effect of Supplementing Boar Semen Cryopreservation Media with Different Avian Egg Yolk Types on in Vitro Post‐thaw Sperm Quality. Reprod. Dom. Anim. 41: 68–73.
BIANCHI, I., CALDERAM, K., MASCHIO, EF, MADEIRA, EM, DA ROSA ULGUIM, R., CORCINI, CD, BONGALHARDO, DC, CORREA, EK, LUCIA JR, T., DESCHAMPS, JC, AND CORREA, MN 2008. Evaluation of amides and centrifugation temperature in boar semen cryopreservation. Theriogenology 69: 632–638.
BUCAK, MN, AND UYSAL, O., 2008. The role of antioxidants in freezing of Saanen goat semen. Indian Vet. J. 85: 148-150.
DIDION, BA, DOBRINSKY, JR, GILES, JR, AND GRAVES, CN, 1989. Staining procedure to detect viability and true acrosome reaction in spermatozoa of various species. Gamete Res. 22: 51–57.
DI SANTO, M., TAROZZI, N., NADALINIW, M., AND BORINI, A. 2012. Human sperm cryopreservation: Update on techniques, effect on DNA integrity, and implications for art. Adv. Urol. 2012: 854837 (12 pages).
EL-BADRY, DA, ABEER M. ANWER, AND RAWASH, ZM 2014. Effect of different concentrations of sodium dodecyl sulfate, egg yolk and glycerol on the freezability and DNA integrity of Arabian stallion spermatozoa. Assiut Vet. Med. J. 60:29-37.
EL-HARAIRY, MA, EID, LN, ZEIDAN, AEB, ABD EL-SALAAM, A.M., EL-KISHK, AND MAM 2011. Quality and fertility of the frozen-thawed bull semen as affected by the different cryoprotectants and glutathione levels. J. Am. Sci. 7: 791–801.
EL-SHARAWY, ME, EL-SEIFY, EM HUSSIEN, A.M., EL-SHERBIENY, M., EL-AND SHAMAA, IS 2015. Effect of Duck Egg Yolk on Cryopreservation and Fertility of Egyptian Buffalo Bull Semen. International J. Agri. Innovations & Res. 3:2319-2437.
FRASER, L., 2004. Structural damage to nuclear DNA in mammalian spermatozoa: its evaluation techniques and relationship with male infertility. Polish J. Vet. Sci. 7: 311-321.
GAO, D., AND CRITSER, JK, 2000. Mechanisms of Cryoinjury In Living Cells. Ilar, J. 41: 187-196.
GARCÍA, BM, FERRUSOLA, CO, APARICIO, IM, MIRO-MORAN, A., RODRIGUEZ, A.M., BOLAOS, JG, FERNANDEZ, LG, DA SILVA, CB, MARTINEZ, HR, TAPIA, JA, AND PENA, FJ 2012. Toxicity of glycerol for the stallion spermatozoa: effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential. Theriogenology 77:1280-1289.
GHALLAB, A.M., ABOU-AHMED, MM, FADL, A.M., EL-BADRY, DA, SHAHAT, A.M., AND MOAWAD, AR 2019. Optimization of the protocol for cryopreservation of Arabian stallion spermatozoa: effects of centrifugation, semen extenders and cryoprotectants. CryoLetters 40: 129-138.
GILMORE, J., LIU, J., GAO, D., AND CRITSER, J. 1997. Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa. Hum. Reprod. 12: 112–118.
GUERRERO, CA, 2006. Cryopreservation and intracytoplasmic sperm injection with bovine epididymal spermatozoa (PhD Thesis). Fac Agric and Mechanical College, Louisiana State University; Mexico.
HAMMERSTEDT, RH, GRAHAM, JK, AND NOLAN, JP, 1990. Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11: 73–88.
HERBOWO, MT, ARIFIANTINI, RI, KARJA, NWK, AND SIANTURI, RG 2019. Cryopreservation of Swamp Buffalo Semen in Skim Milk Yolk-based Diluent with Two Different Cryoprotectants. Tropical An.al Sci. J. 42(1):13-18.
HUGHES, CM, LEWIS, SE, MCKELVEY-MARTIN, V., AND THOMPSON, WA, 1996. Comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod. 2: 613–619.
HU, JH, LI, QW, ZAN, LS, JIANG, ZL, AN, JH, AND WANG, LQ. 2010. The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing-thawing. Anim. Reprod. Sci. 117:11-17.
JEYENDRAN, R. S., VANDER-VEN, HH, PEREZ-PELAEZ, M., CRABO, BG, AND ZANEVLD, LJD, 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. J. Reprod. Fertil. 70: 219-228.
KHALIFA, TAA, 2001. Effect of some antioxidants on the viability of preserved buffalo and ram semen. PhD Thesis, Fac. Vet. Med. Cairo Univ.
KUMAR, D., KUMAR, P., SINGH, P., YADAV, SP, AND YADAV, PS 2016. Assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes. Cytotechnology 68: 451-458.
LEBOEUF, B., RESTALL, B., AND SALAMON, S. 2000. Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci. 62: 113-141.
LEI, SU, XILONG, LI, JIEXIAQUAN, SY, YAHUI, LI, XIECHAO, HEX, AND IANGHUI, T. 2007. A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm Anim. Reprod. Sci. 104: 212-219.
MILOVANOV, VK 1962. Biology of Reproduction and Artificial Insemination of Farm Animals. Monograph. Selkohz. Lit. J. and Plakatov, Moscow.
MOFFET, PD, BRUEMMER, JE, CARD, C., AND SQUIRES, E.L. 2003. Comparison of dimethylformamide and glycerol for cryopreservation of equine spermatozoa. In: Proceedings Society for Theriogenology Annual Conference, 2003, Columbus, OH. Montgomery, AL: pp. 42 (abst.).
MOTA-FILHO, AC, TELES, CHA, JUCA, RP, CARDOSO, JFS, UCHOA, DC CAMPELLO, CC, SILVA, AR, AND SILVA, LDM 2011. Dimethylformamide as a cryoprotectant for canine semen diluted and frozen in ACP-106C. Theriogenology 76:1367-1372.
MOUSTACAS, VS, CRUZ, BC, VARAGO, FC, MIRANDA, DA, LAGE, PG, AND HENRY. M. 2011. Extenders containing dimethylformamide associated or not with glycerol are ineffective for ovine sperm cryopreservation. Reprod. Dom. Anim. 46:924-925.
PUGH, PA, TERVIT, HR, AND NIEMANN, H. 2000. Effects of vitrification medium composition on the survival of bovine in vitro produced embryos, following in straw-dilution, in vitro and in vivo following transfer. Anim. Reprod. Sci. 58: 9-22.
PURDY, PH 2006. A review: on goat sperm cryopreservation, Small Rum. Res. 63: 215-222.
RASUL, Z., AHMED, N., AND ANZAR. M. 2007. Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm. Theriogenology 68:813–819.
REDDY, NSS, MOHANARAO, GJ AND ATREJA, SK 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci. 119: 183-190.
ROHILLA, RK, TULI, RK, AND GOYAL, RL 2005. Comparative study of the effects of cryoprotective agents in freezing Murrah buffalo bull semen. Ind. J. Vet. Res. 14: 37–43.
ROYERE, D., BARTHELEMY, C., HAMAMAH, S., AND LANSAC, J. 1996. Cryopreservation of spermatozoa: A review. Hum. Reprod. 2: 553–559.
SAID, TM, GAGLANI, A., AND AGARWAL, A. 2010. Implication of apoptosis in sperm cryoinjury. Reprod. Biomed. 21: 456–462.
SHAHVERDI, A., RASTEGARNIA, A., AND TOPRAGGALEH, TR 2014. Effect of extender and equilibration time on post-thaw motility and chromatin structure of buffalo (Bubalus bubalis) bull spermatozoa. J. Cell 16: 279-288.
SIANTURI, RG, PURWANTARA, B., SUPRIATNA, I., AMROZI, I., AND SITUMORANG, P. 2012. Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen. JITV. 17: 169-178.
SNEDECOR, GW, AND COCHRAN, WG 1989. Statistical Methods. 8th Ed. Iowa State Univ. Press, Ames, IA, USA.
SU, L., Li, X., QUAN, J., YANG, S., LI, Y., HE, X., AND TANG, X. 2008. A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Anim. Reprod. Sci. 104: 212–219.
SWELUM, AA, MANSOUR, HA, ELSAYED, AA, AND AMER, AH 2011. Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extenders. Theriogenology 76: 833-842.
TASDEMIR, U., BUYUKLEBLEBICI, S., TUNCER, PB, COSKUN, E., OZGURTAS, T., AYDIN, FN, BUYUKLEBLEBICI, O., AND GURCAN, IS 2013. Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology 66: 38–42.
VAFAEI, F., KOHRAM, H., ZAREH-SHAHNE, A., AHMAD, E., AND SEIFI-JAMADI, A. 2019. Influence of Different Combinations of Permeable and Non-permeable Cryoprotectants on the Freezing Capacity of Equine Sperm. J. Equine Vet. Sci. 75: 69.
VIDAMENT. M. 2005. French field results (1985-2005) on factors affecting fertility of frozen stallion semen. Anim. Reprod. Sci. 89:115-134.
WAHEED, S., AHMAD, N., JAMIL-UR-RAHMAN, H., YOUNIS, M., AND IQBAL, S. 2012. Evaluation of duck egg yolk for the cryopreservation of Nili-Ravi buffalo bull semen. Anim. Reprod. Sci. 131: 95–99.
WEBB, GW, BURRIS, CL, HARMON, SE, AND BAKER, RH, 2011. Effects of egg yolk source on the cryopreservation of stallion spermatozoa. J. Equine Vet. Sci. 31: 166–173.
WUNDRICH, K., PAASCH, U., LEICHT, M., AND GLANDER, HJ 2006. Activation of caspases in human spermatozoa during cryopreservation – an immunoblot study. Cell Tissue Bank, 7: 81-90. | ||
|
Statistics Article View: 487 PDF Download: 923 |
||
