Elkhateeb, S., Mahmoud, A., Ibrahem, N. (2020). Protective Role of Quercetin in Hippocampus Inflammatory and Oxidative Damage Induced By Monosodium Glutamate in Rats. test, 28(2), 15-31. doi: 10.21608/mjfmct.2020.31690.1013
Shereen Elkhateeb; Abeer Mahmoud; Nahla Ibrahem. "Protective Role of Quercetin in Hippocampus Inflammatory and Oxidative Damage Induced By Monosodium Glutamate in Rats". test, 28, 2, 2020, 15-31. doi: 10.21608/mjfmct.2020.31690.1013
Elkhateeb, S., Mahmoud, A., Ibrahem, N. (2020). 'Protective Role of Quercetin in Hippocampus Inflammatory and Oxidative Damage Induced By Monosodium Glutamate in Rats', test, 28(2), pp. 15-31. doi: 10.21608/mjfmct.2020.31690.1013
Elkhateeb, S., Mahmoud, A., Ibrahem, N. Protective Role of Quercetin in Hippocampus Inflammatory and Oxidative Damage Induced By Monosodium Glutamate in Rats. test, 2020; 28(2): 15-31. doi: 10.21608/mjfmct.2020.31690.1013

Protective Role of Quercetin in Hippocampus Inflammatory and Oxidative Damage Induced By Monosodium Glutamate in Rats

Mansoura Journal of Forensic Medicine and Clinical Toxicology
Article 2, Volume 28, Issue 2, July 2020, Pages 15-31    PDF (1.67 M)
Document Type: Original Article
DOI: 10.21608/mjfmct.2020.31690.1013
Authors
Shereen Elkhateeb* 1; Abeer Mahmoud2; Nahla Ibrahem3
1Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Egypt.
2Forensic Medicine and Clinical Toxicology Department Faculty of Medicine; Zagazig University, Egypt
3Histology and Cell Biology Faculty of Medicine; Zagazig University, Egypt
Abstract
The aim of the current study was to investigate the possible protective role of quercetin against monosodium glutamate (MSG) induced oxidative stress and inflammation on the brain hippocampus of adult male albino rats. Thirty adult male albino rats were equally divided into 5 groups; Group I (control), group II received1 ml/ kg/ day of 0.5% of Dimethyl sulfoxide (DMSO), group III received 50 mg of quercetin/ kg/ day, group IV received1.6 g/ kg/ day of MSG, group V received quercetin and after one hour, rats received MSG as previously mentioned. All treatments were given orally for 8 weeks. Then rats were anesthetized, sacrificed and specimens from the brain hippocampus were taken for biochemical and histological studies. MSG exposure showed deleterious effects on Y-maze discrimination learning and memory, significantly increased tissue malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), interleukin- 1 beta (IL-1β), tumor necrosis factor-α (TNF-α), glutamate level and acetyl cholinesterase (AChE) activity with significant depletions of catalase (CAT) activities and reduced glutathione (GSH) content. This was accompanied with alteration in the structure of the brain hippocampus. Co-administration of quercetin along with MSG ameliorated the above mentioned effects. The results of this study have shown that quercetin has protective effects against MSG-induced toxicity in rat brain hippocampus.

Highlights
  • MSG induced lipid peroxidation, increased proinflammatory cytokines, glutamate level and AChE activity, decreased antioxidant defense system in brain hippocampus.
  • MSG induced hippocampal neuronal cell damage, had deleterious effects on Y-maze discrimination learning and memory.
  • Quercetin attenuated MSG induced brain hippocampus toxicity by prevention of oxidative stress and inflammation.
  • Quercetin attenuated the neuronal death in the brain hippocampus and improved learning and memory in Y-maze discrimination learning and memory.
Keywords
Monosodium glutamate; Quercetin; Brain hippocampus; Molecular mechanism
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