(2017). Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma. test, 20(2), 113-118. doi: 10.21608/ajdsm.2017.107498
. "Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma". test, 20, 2, 2017, 113-118. doi: 10.21608/ajdsm.2017.107498
(2017). 'Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma', test, 20(2), pp. 113-118. doi: 10.21608/ajdsm.2017.107498
Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma. test, 2017; 20(2): 113-118. doi: 10.21608/ajdsm.2017.107498

Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma

Al-Azhar Journal of Dental Science
Article 3, Volume 20, Issue 2, April 2017, Pages 113-118    PDF (992.65 K)
Document Type: Original Article
DOI: 10.21608/ajdsm.2017.107498
Abstract
Theaim of thecurrentstudywas toevaluate theultrastructural illustrationofapoptosis inducedbygold nanoparticles (AuNPs)conjugatedwithcisplatinand laser irradiationon7,12- Dimethylbenz(a)anthracene (DMBA) inducedhamsterbuccalpouches{HBP(s)}carcinoma.Materialand methods:Theexperimentalanimalsusedin the currentstudywerefifty fivegoldensyrianhamstersweighting80-129g,andobtainedfrom theanimalhouse,Cairo University.Theexperimentalanimalsweredividedintotwomaingroups:GroupA(normalgroup)5hamsterswere taken,nottreatedandservedascontrol.GroupB: Theremaining animals (50hamsters)weretreatedwith thesame procedure:HBPswerepainted for18 weekswith DMBA, 3 timesaweekon alternativedays,then the hamsters were randomly dividedintothefollowing fiveequalsubgroups(10 hamsters)ineachone.GroupB1( DMBAtreated group), groupB2(DMBA-AuNPstreatedgroup), group B3(DMBA-AuNPs-laser radiation treatedgroup), GroupB4(DMBA- cisplatin-laser radiationtreatedgroup)and groupB5(DMBA-AuNPsconjugatedwith cisplatin-laserradiationtreatedgroup). Aftertermination to the treatment, freshtissuesfromHBPweretrimmedintosmallpiecesinorderto beprocessedandexaminedby transmissionelectronmicroscopy(TEM).Results:comparedto resultsof othergroups, DMBA inducedcarcinoma ingroup B animals.AuNPsconjugatedwithcisplatinand laser irradiationgroupshowedsignsofcellular apoptosis ranging from shrinkageofthenuclei,degenerationofthenuclearmembrane to totalcellautolysis. Theother remaninig groupsdidn`tshowanysignsofapoptosis.Conclusions: AuNPs inconjunction with cisplatinandlaserirradiationillustratedvarious signsofcancercellapoptosiswith TEM imaging.Intra-tumoral applicationofAuNPs inconjunctionwithcisplatinandvisiblelaser irradiationwasconsideredasapromising therapeutic agent inregression ofHBP DMBAinduced carcinoma.
 
Keywords
Keywords: Squamous cell carcinoma; Apoptosis; Cisplatin; Gold nanoparticles
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