EVALUATION OF TWO RAPID DIAGNOSTIC METHODS FOR ROTAVIRUS IN BOVINE FAECES | ||
| Kafrelsheikh Veterinary Medical Journal | ||
| Article 9, Volume 10, Issue 1, April 2012, Pages 137-147 PDF (687.49 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/kvmj.2012.112054 | ||
| Authors | ||
| Hala, A. Salem* 1; Hanaa, A. A. Ghoniem2 | ||
| 1Dept. Of virology, Animal Health Research Institiute, Dokki, Giza | ||
| 2Animal Reproduction Resarch Insitute, Al- Haram, Giza | ||
| Abstract | ||
| Rotaviral infections are common cause of economically important calf diarrhea through out the world, Specific, rapid and accurate diagnosis are required to control the disease and animal loss, dipstick (imunnochromatographical test) and enzyme linked immunosorbent assay (ELISA) were used for detection of rotavirus in fecal samples from fifty diarrheic calves were collected from private farms of Alexandria Governorate Bovine Rota virus was detected in 30% of sample which collected from the affected animals using dipstick and ELISA- Dipstick is economical, specific, rapid, easy to use, easy to read and doesn't require equipments or skilled personnel and can be used as alternative for ELISA in under developed countries, small number of daily samples and in laboratories lack. ELISA can detect small amounts of bovine rotaviral particles in faeces, can be completed in less than 4 hours and can be used as routine diagnostic for screening of large numbers of specimens | ||
| Keywords | ||
| Rotaviral infection; imunnochromatographical test; ELISA | ||
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