EFFECT OF CYTOKININ TYPE AND CONCENTRATION ON THE MULTIPLICATION RATE OF SOME ORNAMENTAL PLANTS | ||
| Journal of Plant Production | ||
| Article 14, Volume 34, Issue 6, June 2009, Pages 6689-6698 PDF (697.77 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2009.118647 | ||
| Authors | ||
| A. W. Sayed1; M. A. Khafagy2; A. M. Abo El-Kheer2; F. M. Saadawy1 | ||
| 1Ornamental plants Dept., Hort. Res. Inst., A.R.C. | ||
| 2Agric. Botany Dept., Faculty of Agriculture, Mansoura University | ||
| Abstract | ||
| This study was carried out in the Tissue Culture Laboratory of Zohriya Botanical Garden, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2007-2009 to establish an applicable protocol for the rapid micropropagation of Clerodendrum glabrum and Mussaenda luteola. The tabulated data showed that, in the establishment stage, Clorox concentration had a significant effect on survival percentage of both applying Clorox at 15%, value of survival percentage of both plants explants rose to the significantly highest level. On the other hand, in the multiplication stage, cytokinin type and concentration had a significant effect on the number of shoots and shoot length. In both plants, the significantly highest number of shoots was found on medium supplemented with BAP at 1 ppm, while all media free of cytokinins of any type (the control treatment) resulted in the significantly lowest number of shoots. On the contrary, the untreated control shoots were significantly the longest, while the shortest ones were those treated with BAP. The interaction between cytokinin type and concentration had a significant effect on the number of leaves of Clerodendrom glabrum only. However, the response of the two plants was somewhat contradictory to each other. The highest number of leaves was produced by the untreated control Mussaenda and Clerodendron shoots treated with BAP at 3 ppm, while the lowest number belonged to Mussaenda shoots treated with TDZ at 2 ppm and the shoots grown on the untreated control media. | ||
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