Rashad, N., said, N., Ebaid, A., abdul maksood, R., kadry, H., Ibrahim, N. (2020). Association between interleukin -4 gene polymorphism with the risk and disease activity of Egyptian patients with systemic lupus erythematosus. test, 29(1.2), 102-109. doi: 10.21608/zumj.2020.44123.1954
Nermeen Rashad; nora said; Amany M. Ebaid; rehab abdul maksood; heba kadry; Neveen Ibrahim. "Association between interleukin -4 gene polymorphism with the risk and disease activity of Egyptian patients with systemic lupus erythematosus". test, 29, 1.2, 2020, 102-109. doi: 10.21608/zumj.2020.44123.1954
Rashad, N., said, N., Ebaid, A., abdul maksood, R., kadry, H., Ibrahim, N. (2020). 'Association between interleukin -4 gene polymorphism with the risk and disease activity of Egyptian patients with systemic lupus erythematosus', test, 29(1.2), pp. 102-109. doi: 10.21608/zumj.2020.44123.1954
Rashad, N., said, N., Ebaid, A., abdul maksood, R., kadry, H., Ibrahim, N. Association between interleukin -4 gene polymorphism with the risk and disease activity of Egyptian patients with systemic lupus erythematosus. test, 2020; 29(1.2): 102-109. doi: 10.21608/zumj.2020.44123.1954

Association between interleukin -4 gene polymorphism with the risk and disease activity of Egyptian patients with systemic lupus erythematosus

Zagazig University Medical Journal
Article 563, Volume 29, Issue 1.2, January 2023, Pages 102-109    PDF (445.05 K)
Document Type: Original Article
DOI: 10.21608/zumj.2020.44123.1954
Authors
Nermeen Rashad1; nora said* 2; Amany M. Ebaid3; rehab abdul maksood4; heba kadry5; Neveen Ibrahim6
1Internal medicine, faculty of medicine, zagazig university
2clinical pathology,faculty of medicine departement,zagazig university
3Rheumatology and Rehabilitation department, faculty of medicine, Zagazig university
4Medical Biochemistry Department
5Medical Microbiology & Immunology Department
6Internal Medicine Department
Abstract
Background: Cytokines are a heterogeneous group of molecules organizing different processes of the immune response. Interleukin -4 (IL-4) modulates various immune functions. It plays a vital role in the development of systemic lupus erythematosus (SLE). We aimed to explore the correlation of IL-4 C-589T (rs2243250) gene polymorphism with SLE and its association with disease activity.
Method: This study included 100 SLE patients and 90 healthy controls. Disease activity was evaluated by Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Samples from all participants were analyzed for IL-4 589 genotyping by restriction fragment length polymorphism (RFLP) and serum IL-4 level by enzyme linked immunosorbent assay (ELISA).
Results: IL-4 serum level was significantly lower in patients with SLE (3.14±0.782pg/ml) compared to control (13.82±4.85 pg/ml) (p < 0.001), there was a significant negative correlation between IL-4 serum level and SLEDAI score. CT genotype distribution was significantly lower in SLE patients than controls [OR (95% CI) 0.209 (0.035- 1.246), P < 0.05*]. The frequency of the IL-4 589 T allele was 87% (174 out of 200) in the SLE group compared to 73.9% (133 out of 180) in the controls [OR (2.365 (1.392-4.016), P< 0.01.
Conclusion: CT genotype and T allele of IL-4 C-589T (rs2243250) gene can be a risk factor for SLE. Serum IL-4 level significantly correlated with SLEDAI score
Keywords
systemic lupus erythematosus; SLEDAI; IL-4 589
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