Photoresponsive Production of Hyaluronic Acid from Streptococcus equi under Chemical Mutants | ||
| Egyptian Journal of Botany | ||
| Article 71, Volume 61, Issue 2, August 2021, Pages 415-424 PDF (1.3 M) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2020.47681.1578 | ||
| Authors | ||
| Yasser Attia* 1; Eman Mohamed Mahmoud2; Amany Khalifa3; Mohamed Kobasi4, 5; Mahmoud Sadik6 | ||
| 1National Institute of Laser Enhanced Sciences (NILES), Cairo University, 12613 Giza, Egypt | ||
| 2Microbial Biotechnology Department, Genetic Engineering Division, National Research Centre, Giza 12622, Egypt | ||
| 3National Institute of Laser Enhanced Sciences, Cairo University, Giza 12613, Egypt | ||
| 4Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt | ||
| 5Department of Chemistry, Faculty of Science, Taif University, 21974, Saudi Arabia | ||
| 6Microbiology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt | ||
| Abstract | ||
| THE TRADITIONAL methods for hyaluronic acid (HA) production are costly and require extensive purification of the basic product. These techniques are based on animal extractions and the fermentation from micro-organisms. This research aims to develop a new, improved method for the stable HA production by irradiation Streptococcus equi (S. equi) to low-power UV light under the ethyl methanesulfonate (EMS) influence as a chemical mutagenic agent as well as certain amino acids (L-glutamic acid, L-aspartic acid, L-arginine, L-glycine, and L-lysine) for biosynthesizing metal nanoparticles. The results clearly show the steady development of the control HA (0.045g/L) to 0.25, and 0.27g/L during UV light (254nm) exposure for four minutes and treatments of 25μg/mL (EMS) and amino acids (glutamic acid and lysine). Under these conditions, the incubation of S. equi resulted in an increase in the HA yield by factors of 5.55 and 6, respectively. The molecular mass and chemical analysis of the obtained HA showed considerable similarities compared to the standard sample. The obtained HA was utilized as a capping agent for the biosynthesis of silver (13.0±2.9nm), gold (21.8±1.9nm), and ultra-small zero-valent iron (4.1±0.3nm) nanoparticles. This method provided ample achievement and is effective in HA production. | ||
| Keywords | ||
| Amino Acids; Hyaluronic acid; Mutants; Nanomaterials; Streptococcus equi; UV-photoproduction | ||
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