Insecticidal Activity, Growth Inhibitory and Biochemical Effects of Plant Lectins and Bacillus thuringiensis var. kurstaki against the Pink Bollworm, Pectinophora gossypiella | ||
| Alexandria Science Exchange Journal | ||
| Article 2, Volume 42, JANUARY- MARCH - Serial Number 1, January 2021, Pages 11-20 PDF (334.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asejaiqjsae.2021.144238 | ||
| Author | ||
| Dalia A. El-Deeb* | ||
| Central Agricultural Pesticides Laboratory, Sabahia Station, Alexandria Egypt. | ||
| Abstract | ||
| Insecticidal activity and sublethal effects of white kidney bean and soybean lectins compared to Bacillus thuringiensis var. kurstaki were evaluated against pink bollworm (PBW), Pectinophora gossypiella (Saunders). The in vivo effects of these lectins on the total proteases and α-amylase activities of PBW were also carried out. Results revealed that, B. thuringiensis (LC50 = 61.7 and 36.1 µg / g diet) was approximately 4.7 and 4.8 times more toxic than kidney bean lectins (LC50 = 289.2 and 175.0 µg / g diet) and 2.2 times more toxic than soybean lectins (LC50 = 134.3 and 81.2 µg / g diet) after 5 and 6 days of treatment on the 2nd instar larvae. The LC25 equivalent concentration of B. thuringiensis and soybean lectins achieved the highest reduction of larval weight (11.9 and 13.0 mg / larva compared to 27.1 mg / larva in control) after 9 days of treatment. While kidney bean and soybean lectins at LC10 and LC25 increased the average time to pupation of PBW, B. thuringiensis at LC25 decreased the average time to pupation compared to control. All treatments significantly decreased the pupal mean weight and adult emergence rates compared to control. Kidney bean lectins, soybean lectins and B. thuringiensis at LC25 reduced the average number of eggs laid / female (fecundity) to 35.2, 31.5 and 34.5, respectively, compared to 89.0 in control. In addition, %egg hatch (fertility) significantly decreased to 53.8, 51.7 and 49.1% in kidney bean lectins, soybean lectins and B. thuringiensis LC25 treatments compared to 93.0% in control. Kidney bean and soybean lectins inhibited the activity of total proteases and α-amylase. Results of the present study suggest that kidney bean and soybean lectins can be used as suitable alternatives for B. thuringiensis in integrated management programs of pink bollworm. | ||
| Keywords | ||
| Pink bollworm; Plant lectins; Bacillus thuringiensis; sublethal effects | ||
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