CELLULASE PRODUCTION BY LOCAL BACTERIA ISOLATED FROM TAIF IN SAUDI ARABIA | ||
| Arab Universities Journal of Agricultural Sciences | ||
| Article 11, Volume 19, Issue 1, March 2011, Pages 163-170 PDF (394.57 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajs.2011.14643 | ||
| Authors | ||
| A Sohair* 1; Abozaid Abeer A.2; Hussein Nemmat A.3; Al-salemi Fawzia A.4 | ||
| 1Dept. Microbiol., Fac. Agric., Ain Shams Univ., Egypt & Biol. Dept., Fac. Sci., Taif Univ., Saudi Arabia | ||
| 2Agric. Res. Cen.,Cairo, Egypt & Biol. Dept., Fac. Sci., Taif Univ., Saudi Arabia | ||
| 3Dept. Botany, Fac. Sci., Assiut Univ., Egypt & Biol. Dept., Fac. Sci., Taif Univ., Saudi Arabia | ||
| 4Biol. Dept., Fac. Sci., Taif Univ., Saudi Arabia | ||
| Abstract | ||
| Among 20 bacterial isolates isolated from the soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia,two isolates had high efficacy in producing cellulase enzyme. They belonged to genus Bacillus (Bacillus 8 & 17). Some factors such as carbon source and wheat bran as a raw material, nitrogen source, pH and incubation temperature were investigated. Results indicated that CMC and cellulose were the most effective as they enhanced cellulases production .Sodium nitrate and ammonium chloride were the best nitrogen sources for cellulases production. Initial pH 7.0 was found to be optimal for growth and cellulase production. Incubation temperatures at 25 - 40ºC achieved high cellulases production by the two isolates. | ||
| Keywords | ||
| Cellulase production; Bacillus sp | ||
|
Supplementary Files
|
||
|
Statistics Article View: 206 PDF Download: 292 |
||
