Bessa, S., Ali, E., Gamal, D., Makhlouf, H., Mohamed, T. (2020). Erythrocyte GST activity in type 2 diabetes with and without nephropathy. test, 42(1), 100-107. doi: 10.21608/djs.2020.147773
Sahar S. Bessa; Ehab M.M. Ali; Doaa M.El Gamal; Heba I. Makhlouf; Tarek M. Mohamed. "Erythrocyte GST activity in type 2 diabetes with and without nephropathy". test, 42, 1, 2020, 100-107. doi: 10.21608/djs.2020.147773
Bessa, S., Ali, E., Gamal, D., Makhlouf, H., Mohamed, T. (2020). 'Erythrocyte GST activity in type 2 diabetes with and without nephropathy', test, 42(1), pp. 100-107. doi: 10.21608/djs.2020.147773
Bessa, S., Ali, E., Gamal, D., Makhlouf, H., Mohamed, T. Erythrocyte GST activity in type 2 diabetes with and without nephropathy. test, 2020; 42(1): 100-107. doi: 10.21608/djs.2020.147773

Erythrocyte GST activity in type 2 diabetes with and without nephropathy

Delta Journal of Science
Article 10, Volume 42, Issue 1, December 2020, Pages 100-107    PDF (676.66 K)
Document Type: Research and Reference
DOI: 10.21608/djs.2020.147773
Authors
Sahar S. Bessa1; Ehab M.M. Ali2; Doaa M.El Gamal2; Heba I. Makhlouf* 2; Tarek M. Mohamed2
1Department of Internal Medicine, Faculty of Medicine, Tanta University, Tanta, Egypt
2Department of Chemistry, Division of Biochemistry, Faculty of Science, Tanta University, Tanta, Egypt
Abstract
Diabetic nephropathy (DN) is the main cause of chronic kidney disease, and represents the most common and serious complication of diabetes. The occurrence and progression of DN are closely related to oxidative stress. Excessive reactive oxygen species (ROS) induced by hyperglycemia are involved in direct oxidation and damage of deoxyribonucleic acid (DNA), proteins, and lipids. Glutathione S-transferases have central roles in the cellular detoxification of a diverse group of exogenous and endogenous harmful compounds. The present study aims to clarify the possible role of erythrocyte glutathione S-transferase activity in type 2 diabetic patients with and without nephropathy.  This study included 60 diabetic patients (20 diabetics with normoalbuminuria, 20 diabetics with microalbuminuria, 20 diabetics with macroalbuminuria) and 20 healthy volunteers as a control group. Glutathione S- transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, and Reduced glutathione (GSH) level were significantly lower in diabetic patients with and without nephropathy as compared to control. Malondialdehyde (MDA) level was significantly higher in diabetic patients with and without nephropathy as compared to control. GST, SOD, GPx, CAT, GSH and MDA were positively correlated with estimated glomerular filtration rate and negatively correlated with albumin creatinine ratio. It was concluded that erythrocyte GST may be used as a biomarker to differentiate between different groups of nephropathy.
Keywords
Diabetic nephropathy; Oxidative stress; Glutathione S-transferase
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