Faridi, B., Pourmirza, A., Safaralizadeh, M., Taghaddosi, M. (2011). The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad. test, 4(2), 47-53. doi: 10.21608/eajbsa.2011.15164
Bahram Faridi; Ali Asghr Pourmirza; Mohammad Hossien Safaralizadeh; Mohammad Vali Taghaddosi. "The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad". test, 4, 2, 2011, 47-53. doi: 10.21608/eajbsa.2011.15164
Faridi, B., Pourmirza, A., Safaralizadeh, M., Taghaddosi, M. (2011). 'The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad', test, 4(2), pp. 47-53. doi: 10.21608/eajbsa.2011.15164
Faridi, B., Pourmirza, A., Safaralizadeh, M., Taghaddosi, M. The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad. test, 2011; 4(2): 47-53. doi: 10.21608/eajbsa.2011.15164

The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 6, Volume 4, Issue 2, December 2011, Pages 47-53    PDF (98.44 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2011.15164
Authors
Bahram Faridi1; Ali Asghr Pourmirza1; Mohammad Hossien Safaralizadeh1; Mohammad Vali Taghaddosi2
1Department of Entomology, Faculty of Agriculture, Urmia University, Urmia, Iran
2Agricultural and Natural Resources Research Center of Zanjan
Abstract
The susceptibility of Leptinotarsa
decemlineata (Say) adults to spinosad was determined by leaf disk and glass
jar techniques under laboratory conditions. The mortalities of the treated
adults were recorded 72 h post treatments. LC50s values in leaf disk
and glass jar methods for adults were 98.50 and 108.34 mg a.i/L, respectively.  A significant linear relationship between LC50
values of individual population across test methods was detected (r = 0.93,
P < 0.05). Both bioassay
techniques were suitable for monitoring resistance to spinosad in Colorado
potato beetle adult populations. Glass jar technique, however, exhibited less
variability in LC50 estimates and showed a higher degree of
sensitivity than the leaf disk method. Filter paper and leaf disk techniques
for larvae were two bioassay methods used to determine spinosad susceptibility
in L. decemlineata population. Both bioassay techniques exhibited a different
level of susceptibility of the larvae to spinosad; however, the confidence
interval values from filter paper method were narrow than the leaf disk assay
technique. A significant direct relationship between LC50 values of
individual population across test methods was observed (r = 0.96, P < 0.05).  The glass jar and filter paper testing
methods are simple and sensitive test techniques for measuring susceptibility
of Colorado potato beetle adults and larvae to spinosad, respectively. The
efficacy of spinosad can be conserved for a long period of time if it is
judiciously rotated with other suitable insecticides in a spray program and the
number of application is restricted.   
Keywords
: bioassay; Colorado potato beetle; leaf disk; paper disk; toxicity; Spinosad
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