Helal, O., Metwaly, H., Abo Gazia, M., El Desoki, R., el ashmouny, N. (2021). The possible therapeutic Effect of Mesenchymal Stem Cells and Vitamin E on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological and immunohistochemical study. test, 38(1), 294-321. doi: 10.21608/bmfj.2021.24068.1215
Omayma Helal; Hala Metwaly; Maha Abo Gazia; Rania El Desoki; naira ashraf el ashmouny. "The possible therapeutic Effect of Mesenchymal Stem Cells and Vitamin E on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological and immunohistochemical study". test, 38, 1, 2021, 294-321. doi: 10.21608/bmfj.2021.24068.1215
Helal, O., Metwaly, H., Abo Gazia, M., El Desoki, R., el ashmouny, N. (2021). 'The possible therapeutic Effect of Mesenchymal Stem Cells and Vitamin E on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological and immunohistochemical study', test, 38(1), pp. 294-321. doi: 10.21608/bmfj.2021.24068.1215
Helal, O., Metwaly, H., Abo Gazia, M., El Desoki, R., el ashmouny, N. The possible therapeutic Effect of Mesenchymal Stem Cells and Vitamin E on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological and immunohistochemical study. test, 2021; 38(1): 294-321. doi: 10.21608/bmfj.2021.24068.1215

The possible therapeutic Effect of Mesenchymal Stem Cells and Vitamin E on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological and immunohistochemical study

Benha Medical Journal
Article 25, Volume 38, Issue 1, April 2021, Pages 294-321    PDF (2.93 M)
Document Type: Original Article
DOI: 10.21608/bmfj.2021.24068.1215
Authors
Omayma Helal1; Hala Metwaly2; Maha Abo Gazia3; Rania El Desoki1; naira ashraf el ashmouny* 4
1Department of Histology and Cell Biology, faculty of medicine, Benha University, Egypt.
2Department of Clinical Pathology, Faculty of Medicine, Cairo University, Egypt.
3Department of Histology and Cell Biology, Faculty of Medicine- Kafr El Sheikh University. Egypt.
4assistant lecturer of Histology and Cell Biology, Faculty of Medicine, Kafr El Sheikh University, Egypt
Abstract
Background:
Ulcerative colitis (UC) is one of the inflammatory bowel diseases (IBD) that results in recurrent inflammation and ulcers of the colon and rectum.
Aim:
The aim of the study was to explore the possible therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) and vitamin E on experimentally induced UC in rats.
Materials and methods:
Sixty rats were divided into four main groups: group I (control group); group II (UC) consisted of 10 rats that received a single intrarectal injection of 2 ml of 3% acetic acid; group ΙIΙ (10 rats) consisted of rats that received vitamin E three times in a week orally after induction of ulcerative colitis; group ΙV consisted of 10 rats that received a single injection of BM-MSCs after induction of ulcerative colitis ; and group V comprised 10 rats that received both vitamin E orally and BM-MSCs after induction of colitis. Colonic samples were examined using light and fluorescent microscopy.
Results:

Group ΙI showed ulceration, loss of surface columnar epithelium, disturbed crypts architecture with few goblet cells and many cellular infiltrations. Group III showed a picture that was nearly similar to UC group. Group IV showed improvement in colitis. Group V showed a histological picture that was nearly similar to the control group.
Conclusions:
BM-MSCs represent future therapeutic hopes for chronic intestinal inflammatory states through their regenerative and anti-inflammatory effects. The combination of vitamin E and BM-MSCs was more effective and produced an additive effect than using bone marrow derived MSCs alone.
Keywords
Ulcerative colitis; Acetic acid; MSCs; PKH26; Rat colon
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