Biochemical Markers for Acetamiprid and Imidacloprid Neonicotinoid Insecticides Selectivity in the Cotton White Fly, Bemisia tabaci, the Cotton Leafworm, Spodoptera littoralis and Honey Bee, Apis mellifera | ||
| Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||
| Article 6, Volume 9, Issue 3, December 2017, Pages 61-73 PDF (386.28 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsf.2017.17028 | ||
| Authors | ||
| Shimaa Gh. M. Marzouk1; Ali A. El-Sheakh1; Mostafa A. El-Tantawy2; Mohamed- -Bassem A. Ashour2 | ||
| 1Plant Protection Research Institute, Agricultural Research Center, Dokki, Egypt. | ||
| 2Department of Plant Protection, Faculty of Agriculture, Zagazig University, sharkia, Egypt | ||
| Abstract | ||
| Selective toxicity of acetamiprid (Mospilan 20% SP) and imidacloprid (Imaxi 35% SC) neonicotinoid insecticides to Bemisia tabaci, Spodoptera littoralis and Apis mellifera was studied. Assessment of biochemical alterations total proteins, acetylcholinesterase EC3.1.1.7(AChE) activity, cytochrome P450 monooxygenases EC1.14.14.1 (CP450) activity and glutathione-S-transferases EC2.5.1.18 (GST) activity were recorded and discussed as biomarkers for acetamiprid and imidacloprid selective toxicity in the exposed insects . Imidacloprid treatments caused higher reductions in the total protein levels as compared with acetamiprid treatments. The total protein were decreased by 55, 41, 31 and 14 % in A. mellifera thorax, S. littoralis 2nd larval instar, B. tabaci adult and S. littoralis 4th larval instar, respectively. AChE activity was increased by 29% and decreased by 50% in A. mellifera whole body in acetamiprid and imidacloprid treatments, respectively. Moreover in B. tabaci, the enzyme activity was decreased by 9% and increased by 35% respectively after acetamiprid and imidacloprid treatments. The two tested neonicotinoids elevated CP450 activity in B. tabaci adults and S. littoralis 2nd and 4th laraval instars. The highest increase was obtained with imidacloprid treatment in A. mellifera thorax (165%) followed by S. littoralis 2nd larval instar (100%), B. tabaci adults (66%). The obtained results showed that acetamiprid and imidacloprid treatments elevated GST activity respectively by 29 and 1.5 % in B. tabaci adults; 30 and 30% in S. littoralis 4th laraval instar ;88 and 59% in A. mellifera thorax . GST activity was decreased by 18% in S. littoralis 2nd larval exposed to acetamiprid and increased by 18% in imidacloprid treatment. Conclusively, our work suggested that the selective toxicity of the cyanoguanidine neonicotinoid acetamiprid and the nitroguanidine neonicotinoid imidacloprid was based on their chemical structure and metabolic pathways and seemed to be represent a species-specific feature .This was indicated by alterations of the values of total proteins and enzymes specific activity of key enzymes in the exposed target and non target insects . | ||
| Keywords | ||
| neonicotinoid; Selectivity; Biochemical markers; Bemisia tabaci; Spodoptera littoralis; Apis mellifera | ||
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