Assessing Inter and Intra Molecular Genetic Variation of Litter Size in Three Egyptian Goat Breeds Using COI and F-AFLP Markers | ||
| Arab Universities Journal of Agricultural Sciences | ||
| Article 164, Volume 29, Issue 1, April 2021, Pages 341-350 PDF (431.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajs.2021.56289.1326 | ||
| Authors | ||
| Mohamed Reda Ismail Anous* 1; Emanuel Kodit2; Hagar Elhifnawy3; Mohamed Rashed4; Mohamed Sadek5 | ||
| 1Department of Animal Production, Faculty of Agriculture, Ain-Shams University, Cairo, Egypt | ||
| 2Department of Animal Production, Faculty of Agriculture, Ain Shams University,Cairo, Egypt | ||
| 3Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||
| 4Genetics Department, faculty of Agriculture, Ain-Shams university, Cairo, Egypt | ||
| 5Department of Animal Production, Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| establishing adequate utilization of genetic variation in developing livestock breeding programs. Cytochrome oxidase subunit I (COI) gene technique was used in the present study to identify and differentiate the main three Egyptian local goat breeds (i.e. Baladi, Zaraibi and Barki) via DNA barcoding to confirm its species identity and provide valuable DNA sequence source in the nucleotide online database for further studies. Blast (Basic Local Alignment Search Tool) results confirmed samples to be Capra hircus (100%) with no variation among the studied breeds. On the other hand, the Fluorescently Amplified Fragment Length Polymorphism (F-AFLP) technique was applied to assess genetic variation among and within the three breeds for litter size character. F-AFLP analysis of triplicates per breed produced 164 polymorphic loci. At the same time fixed and private bands varied among the three breeds; 47, 17 and 14 bands and 9, 19 and 27 bands for Baladi, Zaraibi and Barki, respectively. Analysis of Molecular Variance (AMOVA) showed 3.8% and 96.1% variance among and within breeds, respectively. Population re-allocation showed that all samples of Baladi breed are outliers, Zaraibi breed one outlier and two hybrids and in Barki breed one hybrid, one outlier and one allocates itself. Private bands in excel filter (using virtual inspection in excel) showed fixed bands of 213bp molecular weight at locus 35 in both Baladi and Zaraibi breeds. These bands considered as genetic marker for litter size trait (i.e. high prolific animals). | ||
| Keywords | ||
| COI sequencing; F-AFLP technique; Egyptian goats; Molecular variance; Litter size | ||
|
Statistics Article View: 423 PDF Download: 381 |
||
