PRELIMINARY STUDY ON THE PRODUCTION OF NITRIC OXIDE AND EXPRESSION OF NITRIC OXIDE SYNTHASE BY CAMEL MACROPHAGES

Assiut Vet. Med. J. Vol. 52 No. 111 October 2006

Dept. of Microbiology, Fac. of Vet. Med., Cairo University

PRELIMINARY STUDY ON THE PRODUCTION OF NITRIC OXIDE AND EXPRESSION OF NITRIC OXIDE SYNTHASE BY CAMEL

MACROPHAGES (With One Table and One Figure)

By M. HASHAD; M. AL DUBAIB*; K. AL KHERIJI*

and SAHER A.GALAL ** Dept. of Vet. Medicine, College of Agriculture and Vet. Med., Qassim Univ. Dept. of Immunity, Amimal Health Research Institute, Dokki, Giza, Egypt.

(Received at 24/9/2006)

دراسة مبدئية لإنتاج أکسيد النيتريک والتعبير الجيني عن أنزيم تمثيل أکسيد

النيتريک لماکروفاج الجمال محمود السيد حشاد،محمود الدبيب،خالد الخريجي

سحر عبد اللطيف جل

من المعروف أن أکسيد النيتريک ينتج بواسطة الماکروفاج المحفزة بواسطة بعض الأنتيجينات أو الميکروبات. في هذه الدراسة تم فصل کرات الدم البيضاء من دم الجمال بواسطة الفيکول وبعد ذلک تم فصل الخلايا وحيدة النواة وهذه الخلايا تم إنمائها إلى الماکروفاج. تم تعريض خلايا الماکروفاج البالغة على متعدد السکريات الدهنية المشتقة من الإيشيرشيا کولای وکذا

کل من أکسيد النيتريک و الحمض النووي المرسل الخاص

أنتيجين جدري الأغنام وتم قياس بإنزيم تمثيل أکسيد النيتريک وقد استطاع کلا الأنتيجينين تحفيز إنتاج أکسيد النيتريک مع کون أنتيجين جدري الأغنام الأقل تحفيزا. وعند إجراء اختبار تفاعل البوليميريز المتسلسل

بإنتاج أکسيد النيتريک المنتج من

بإستخدام بوادئ خاصة بالحمض النووي المرسل الخاص ماکروفاج الجمال أسفرت الدراسة عن منتج بنفس حجم المقابل له في الفئران مما يدل على

بذلک.

ثبات في العامل الوراثي الخاص

SUMMARY

Nitric Oxide is produced by macrophage when activated or invaded by certain antigens or microbes. In the present study, camel peripheral blood leukocytes were obtained by ficoll cushion. Monocytes were separated and grown to macrophages. The mature macrophages were exposed to E. coli LPS as well as sheep pox virus antigen. Nitric oxide

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Conclusively, results obtained in this study indicate that nitric oxide and nitrogenous compound intermediates are produced by activated camel macrophages like macrophages of other animal species. This supports, to a considerable limit, the stability of the microbicidal armatures in different animal species. However, further studies are required to detect production of other armatures such as the active oxygen radicals by camel macrophages. Moreover, the iNOS mRNA was found to be conserved among the species studied so far including camel.

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