Farghaly, L., Sabak, N., El-sherbeny, N. (2007). The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats. test, 26(1), 31-45. doi: 10.21608/ejhm.2007.17778
Lamia M. Farghaly; Nagwan A. Sabak; Naglaa A. El-sherbeny. "The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats". test, 26, 1, 2007, 31-45. doi: 10.21608/ejhm.2007.17778
Farghaly, L., Sabak, N., El-sherbeny, N. (2007). 'The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats', test, 26(1), pp. 31-45. doi: 10.21608/ejhm.2007.17778
Farghaly, L., Sabak, N., El-sherbeny, N. The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats. test, 2007; 26(1): 31-45. doi: 10.21608/ejhm.2007.17778

The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats

The Egyptian Journal of Hospital Medicine
Article 3, Volume 26, Issue 1, January 2007, Pages 31-45    PDF (1.26 M)
Document Type: Original Article
DOI: 10.21608/ejhm.2007.17778
Authors
Lamia M. Farghaly1; Nagwan A. Sabak2; Naglaa A. El-sherbeny3
1Departments of Histology Faculty of Medicine, Suez Canal University
2Biochemistry Faculty of Medicine, Suez Canal University.
3Pharmacology, Faculty of Medicine, Suez Canal University.
Abstract
Aim of the study: This study was conducted to investigate the possible protective effects of L- treptophan "a precursor of melatonin" and alpha lipoic acid against L- arginine-induced experimental acute pancreatitis in albino rats.
Methods: Fourty adult male albino rats (200- 250g) were randomized into 4 groups (n= 10). Group I, the control group was given 0.9% saline intraperitoneally (i.p). Group II, was given 500 mg/100g L-arginine (i.p) as a single dose to induce acute pancreatitis. Group III: was given 250mg/kg L-tryptophan (i.p) 30 min prior to L- arginine injection. Group IV: was given 50mg/kg alpha lipioc acid(i.p) 30 min prior to L-arginine. Before scarifice, blood samples were obtained from all groups to assay serum amylase and interleukin 6. Animals were sacrificed after 6 hours. For the histopathological study, pancreatic tissue was prepared for histological (H&E, PAS) histochemical ( Tween stain for lipases) and immunohistochemical ( Bax stain for apoptosis) techniques. Both qualitative and quantitative analyses were done to assess the degree of acinar cells affection
Results: It was revealed that serum amylase and interleukin 6 in group II rose rapidly. Microscopically, severe acinar cells degeneration, interstitial edema, diffuse bleeding and inflammatory infiltration were demonstrated. These changes were markedly improved with the administration of both L- tryptophan and alpha lipoic acid.
Conclusion: It was concluded that both L- tryptophan & alpha lipoic acid reduced the effects of L-arginine-induced acute pancreatitis with better protection achieved by L-tryptophan administration.
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