Abbès, S., Abbès, J., Ouanes, Z., Bacha, H., Othman, O., Abdel-Wahhab, M., Oueslati, R. (2006). Hydrated Sodium Calcium Aluminsilicate Protects Against Genotoxic Effects In Zeralenone-Treated Balb/C Mice. test, 22(1), 49-59. doi: 10.21608/ejhm.2006.18025
Samir Abbès; Jalila Ben Salah- Abbès; Zouhour Ouanes; Hassan Bacha; Omar Othman; Mosaad A. Abdel-Wahhab; Ridha Oueslati. "Hydrated Sodium Calcium Aluminsilicate Protects Against Genotoxic Effects In Zeralenone-Treated Balb/C Mice". test, 22, 1, 2006, 49-59. doi: 10.21608/ejhm.2006.18025
Abbès, S., Abbès, J., Ouanes, Z., Bacha, H., Othman, O., Abdel-Wahhab, M., Oueslati, R. (2006). 'Hydrated Sodium Calcium Aluminsilicate Protects Against Genotoxic Effects In Zeralenone-Treated Balb/C Mice', test, 22(1), pp. 49-59. doi: 10.21608/ejhm.2006.18025
Abbès, S., Abbès, J., Ouanes, Z., Bacha, H., Othman, O., Abdel-Wahhab, M., Oueslati, R. Hydrated Sodium Calcium Aluminsilicate Protects Against Genotoxic Effects In Zeralenone-Treated Balb/C Mice. test, 2006; 22(1): 49-59. doi: 10.21608/ejhm.2006.18025

Hydrated Sodium Calcium Aluminsilicate Protects Against Genotoxic Effects In Zeralenone-Treated Balb/C Mice

The Egyptian Journal of Hospital Medicine
Article 5, Volume 22, Issue 1, January 2006, Pages 49-59    PDF (332.61 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2006.18025
Authors
Samir Abbès1; Jalila Ben Salah- Abbès1; Zouhour Ouanes2; Hassan Bacha2; Omar Othman1; Mosaad A. Abdel-Wahhab3; Ridha Oueslati1
1Laboratory of Environmental Immunology Microbiology and Cancerology, Faculty of Sciences Bizerte, 7021 Zarzouna, Tunisia
2Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir , Tunisia
3Food Toxicology & Contaminants Dept. Nationa l Research Centre, Dokki, Cairo, Egypt.
Abstract
Zearalenone (ZEN) is a potent estrogenic metabolite mycotoxin produced by some Fusarium species. Few studies have been successfully employed to get rid of the ZEN contamination in foods. This study was conducted to evaluate the ability of hydrated sodium calcium aluminsilicate (HSCAS) to protect Balb/c mice against cytotoxicity and genotoxicity induced by ZEN. Mice were divided into nine experimental groups (12 mice/group) included the control group, the olive oil group, the groups treated orally with a single dose of HSCAS at doses level of 400, 600 and 800 mg/kg b.w, the group treated orally with a single dose of ZEN (40 mg/kg b.w), the group treated with ZEN plus HSCAS (400 mg/kg b.w), the group received Colchicin(4mg/kgbw)asapositivecontrolfor micronucleusassayandthegrouptreatedwith mitomycin C (1 mg/kg bw) as a positive control for chromosome aberrations assay. Forty eight hours after treatment, the femur and tibia were dissected out and bone marrow was obtained for different assays. The results showed that ZEN was cytotoxic and genotoxic to Balb/c mice as indicated by the increase in frequencies of polychromatic erythrocytes micronucleated (PCEMN) and chromosomal aberrations in bone marrow cells. The simultaneous administration of HSCAS with ZEN resulted in a decrease of PCEMN number and chromosomal aberrations frequency and increased the polychromatic erythrocytes (PCE) in bone marrow cells compared with the ZEN alone group. It could be concluded that HSCAS itself was safe at different tested doses and efficient in the prevention of ZEN induced clastogenicity in mice at a dose level as low as 400 mg/kg b.w.
 
Keywords
HSCAS; zearalenone; Micronuclei; Chromosome aberrations; prevention
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