IDENTIFICATION OF MUTATION IN THE BEMISIA TABACI (GENN.) PARA SODIUM CHANNEL GENE ASSOCIATED WITH RESISTANCE TO PYRETHROIDS | ||
| Egyptian Journal of Agricultural Research | ||
| Article 14, Volume 88, Issue 1, March 2010, Pages 153-166 PDF (467.56 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2010.180255 | ||
| Authors | ||
| SAUDA F. FARGHALY; HALA M. ABO-YOUSSEF; MOHAMED SENGAB; YAHYA F. GHONEM | ||
| Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, Egypt. | ||
| Abstract | ||
| Mechanism of resistance to lambda-cyhalothrin in highly resistant Bemisia tabaci strain can be explained by the voltage-gated sodium channel which is the primary target site of pyrethroid insecticides. The super knockdown resistance (super-kdr) to pyrethroids is caused by changes at specific sites on the para-type sodium channel protein domain II (IIS4-6). The B. tabaci para-type sodium channel gene was RT-PCR amplified from lab-strain (reference), Parent (first generation after treatment with the insecticide) and generation thirteen after treatment was considered. The mechanisms of resistance to Lambda-cyhalothrin (Karat 20% EC) in a Q biotype, highly resistant Bemisia tabaci strain. Analysis of the sequence of the IIS4-IIS6 region of the para sodium channel gene of lab-strain, Parent and resistant strain (G13) revealed two amino acid replacements compared to that of the SUD-S susceptible strain. One is the leucine to isoleucine substitution at position 925 (L925I) and allele r1-Q1 (GenBank accession no. DQ205206) has identical intron sequences with samples of parent and lab-strain, other is a novel kdr resistant mutation for B. tabaci, a threonine to valine substitution at position (T929V) and alleles r2-Q1 (GenBank accession no DQ205207) has identical intron sequence with G13.. | ||
| Keywords | ||
| Bemisia tabaci; sodium channel gene; insecticide resistance; pyrethroids | ||
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