gouda, S., Ahmed, D., Abo senna, A., Said, E., Abdallah, W., Ibrahiem, N. (2021). Molecular Assessment of DNA Methylation Biomarkers in Egyptian Hepatocellular carcinoma Patients.. test, 38(3), 925-936. doi: 10.21608/bmfj.2021.67156.1395
seham gouda; Dalia Ahmed; Azza Abo senna; Ebada Said; Wafaa Abdallah; Nermeen Ibrahiem. "Molecular Assessment of DNA Methylation Biomarkers in Egyptian Hepatocellular carcinoma Patients.". test, 38, 3, 2021, 925-936. doi: 10.21608/bmfj.2021.67156.1395
gouda, S., Ahmed, D., Abo senna, A., Said, E., Abdallah, W., Ibrahiem, N. (2021). 'Molecular Assessment of DNA Methylation Biomarkers in Egyptian Hepatocellular carcinoma Patients.', test, 38(3), pp. 925-936. doi: 10.21608/bmfj.2021.67156.1395
gouda, S., Ahmed, D., Abo senna, A., Said, E., Abdallah, W., Ibrahiem, N. Molecular Assessment of DNA Methylation Biomarkers in Egyptian Hepatocellular carcinoma Patients.. test, 2021; 38(3): 925-936. doi: 10.21608/bmfj.2021.67156.1395

Molecular Assessment of DNA Methylation Biomarkers in Egyptian Hepatocellular carcinoma Patients.

Benha Medical Journal
Article 178, Volume 38, Issue 3, November and December 2021, Pages 925-936    PDF (528.71 K)
Document Type: Original Article
DOI: 10.21608/bmfj.2021.67156.1395
Authors
seham gouda* 1; Dalia Ahmed2; Azza Abo senna3; Ebada Said4; Wafaa Abdallah3; Nermeen Ibrahiem2
1Clinical and Chemical Pathology Faculty of Medicine, Benha University.
2Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha,Egypt.
3Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha,Egypt
4Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Benha University, Benha, Egypt.
Abstract
BACKGROUND: HCC is a primary tumor of the liver. It is one of the most common malignancies in adults. OBJECTIVE: Assessment of DNA methylation of BMP4, TSPYL5, APC1 and p16 genes in Egyptian hepatocellular carcinoma patients. METHODS: The present study included 180 subjects classified into: group (I) 60 HCC patients, group (II) 60 chronic liver disease patients with chronic HCV infection who were diagnosed by presence of HCV Abs for more than 6 months and quantitative PCR for HCV RNA and group (III) 60 apparently healthy volunteers as control subjects. For all participants, the following was done: Routine lab investigation including CBC, PT, PC, AST, ALT, ALP, albumin, Bilirubin, creatinine, AFP, abdominal ultra sonography, Spiral triphasic CT, and molecular assessement of p16, TSPYL5, ACP1, BMP4 methylation status. RESULTS: Gene expression by RT-PCR technique among studied groups; APC1, BMP4, TSPYL5, and P16 were statistically significant (p < 0.001) in HCC compared to control. CONCLUSIONS: Hypermethylation of p16, ACP1, BMP4, and TSPYL 5 are common events in patients with HCC and could be used as potentially detectable biomarkers in HCC...
Keywords
DNA methylation; DNA biomarkers; Hepatocellular carcinoma; chronic liver disease; HCV
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