El-sherbeney, S., Ahmed, H., El-Tahawy, N., Abdalla, A. (2013). The Possible Ameliorative Effect of Melatonin on Oxidative Stress and Delayed Apoptosis in Rat's Brain Exposed to Deltamethrin. test, 20(1), 38-50. doi: 10.21608/ajfm.2013.19385
Shereen El-sherbeney; Hala Ahmed; Nashwa El-Tahawy; Ahlam Abdalla. "The Possible Ameliorative Effect of Melatonin on Oxidative Stress and Delayed Apoptosis in Rat's Brain Exposed to Deltamethrin". test, 20, 1, 2013, 38-50. doi: 10.21608/ajfm.2013.19385
El-sherbeney, S., Ahmed, H., El-Tahawy, N., Abdalla, A. (2013). 'The Possible Ameliorative Effect of Melatonin on Oxidative Stress and Delayed Apoptosis in Rat's Brain Exposed to Deltamethrin', test, 20(1), pp. 38-50. doi: 10.21608/ajfm.2013.19385
El-sherbeney, S., Ahmed, H., El-Tahawy, N., Abdalla, A. The Possible Ameliorative Effect of Melatonin on Oxidative Stress and Delayed Apoptosis in Rat's Brain Exposed to Deltamethrin. test, 2013; 20(1): 38-50. doi: 10.21608/ajfm.2013.19385

The Possible Ameliorative Effect of Melatonin on Oxidative Stress and Delayed Apoptosis in Rat's Brain Exposed to Deltamethrin

Ain Shams Journal of Forensic Medicine and Clinical Toxicology
Article 5, Volume 20, Issue 1, January 2013, Pages 38-50    PDF (7.87 M)
Document Type: Original Article
DOI: 10.21608/ajfm.2013.19385
Authors
Shereen El-sherbeney1; Hala Ahmed1; Nashwa El-Tahawy2; Ahlam Abdalla3
1Forensic Medicine and Clinical Toxicology department, Faculty of Medicine, Minia University, Minia, Egypt
2Histology department, Faculty of Medicine, Minia University, Minia, Egypt
3Biochemistry department, Faculty of Medicine, Minia University, Minia, Egypt
Abstract
Deltamethrin is a broad spectrum, non- cumulative, fast acting neurotoxic synthetic pyrethroid. The aim of this study was to analyze the neurotoxicity induced by deltamethrin which caused neuro-oxidative stress, histopathological & immunohistochemical changes in adult male Wistar rats and to evaluate the possible ameliorative effect of melatonin. Melatonin (10mg/Kg, once daily), ethanol 99% (1 ml/kg, once daily), corn oil (0.005ml/Kg, once daily), deltamethrin (12.5mg/Kg, once daily), and melatonin (10mg/Kg) + deltamethrin (12.5mg/Kg) combination were given to rats I.P for 8 weeks. By the end of 8th week, rats given deltamethrin alone had significantly higher MDA (malondialdehyde), lower reduced glutathione and catalase levels in the hippocampus and lower ACTH (adrenocortocotrophic hormone) & cortisol hormone levels than the control group. Co- treatment of deltamethrin with melatonin had an ameliorative effect on the previous parameters. Light microscopic investigations revealed that deltamethrin caused marked degenerative changes in the hippocampus & pituitary gland of rats. Mild degenerative changes were observed in rats received deltamethrin + melatonin combination. Immunohistochemical studies revealed that deltamethrin caused marked expression of P-53 (a marker for apoptosis) in the hippocampus & pituitary gland. Mild expression of P-53 was observed in rats received deltamethrin & melatonin combination. Thus, deltamethrin can induce oxidative damage in rat brain & melatonin has an ameliorative effect against this damage.
Keywords
Deltamethrin; Neurotoxicity; histopathology; Immunohistochemistry; Melatonin
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