Astuti, A., Yasir, B., Rahim, A., Natzir, R., Subehan, S., Goto, K., Alam, G. (2022). Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction. test, 65(3), 255-260. doi: 10.21608/ejchem.2021.80171.3972
Ayun Dwi Astuti; Budiman Yasir; Abdul Rahim; Rosdiana Natzir; Subehan Subehan; Kyoko Nakagawa Goto; Gemini Alam. "Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction". test, 65, 3, 2022, 255-260. doi: 10.21608/ejchem.2021.80171.3972
Astuti, A., Yasir, B., Rahim, A., Natzir, R., Subehan, S., Goto, K., Alam, G. (2022). 'Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction', test, 65(3), pp. 255-260. doi: 10.21608/ejchem.2021.80171.3972
Astuti, A., Yasir, B., Rahim, A., Natzir, R., Subehan, S., Goto, K., Alam, G. Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction. test, 2022; 65(3): 255-260. doi: 10.21608/ejchem.2021.80171.3972

Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction

Egyptian Journal of Chemistry
Article 24, Volume 65, Issue 3, March 2022, Pages 255-260    PDF (511.16 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.80171.3972
Authors
Ayun Dwi Astuti1; Budiman Yasir2; Abdul Rahim3; Rosdiana Natzir4; Subehan Subehan5; Kyoko Nakagawa Goto6; Gemini Alam* 7
1Department of Medical Science, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
2Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
3Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar 90245, Indonesia
4Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
5Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar 90245, Indonesia
6School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa 920-1192, Japan.
7Faculty of Pharmacy, Hasanuddin University Indonesia
Abstract
Plectranthus scutellarioides is one of medicinal plants in Indonesia, traditionally known as iler/miana/jawer kotok. Due to a large number of varieties, certain varieties of P. scutellarioides have not been extensively studied. This study focused on the isolations of two phytosterols, stigmasterol and β-sitosterol, which were characterized by various 1D and 2D NMR analyses, from the ethyl acetate extract of P. scutellarioides var. color blaze dark star. Cytotoxicity of the ethyl acetate extract and some selected fractions were evaluated against A549, MDA-MB-231, MCF-7, KB, and KB-Vin cell lines using SRB (Sulforhodamine B) method. Among all tested sample, fractions 15 and 17 have significantly inhibited cell growth with IC50 of 14.3-24.3 µg/mL and 7.3-14.1 µg/mL, respectively.
Keywords
Plectranthus scutellarioides; stigmasterol; β-sitosterol; cytotoxicity
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