AbouRayya, S., Kasem, N., Ibrahim, E., Salim, R., Mahmoud, T. (2022). Fruit Physical, Chemical and Molecular Identification of Three Walnuts (Juglans regia L.) Genotypes Grown in Egypt. test, 65(2), 651-658. doi: 10.21608/ejchem.2021.90859.4326
Sami M AbouRayya; Nabila El Badawy Kasem; Eman Ibrahim; Rasha G Salim; Thanaa Sh.M. Mahmoud. "Fruit Physical, Chemical and Molecular Identification of Three Walnuts (Juglans regia L.) Genotypes Grown in Egypt". test, 65, 2, 2022, 651-658. doi: 10.21608/ejchem.2021.90859.4326
AbouRayya, S., Kasem, N., Ibrahim, E., Salim, R., Mahmoud, T. (2022). 'Fruit Physical, Chemical and Molecular Identification of Three Walnuts (Juglans regia L.) Genotypes Grown in Egypt', test, 65(2), pp. 651-658. doi: 10.21608/ejchem.2021.90859.4326
AbouRayya, S., Kasem, N., Ibrahim, E., Salim, R., Mahmoud, T. Fruit Physical, Chemical and Molecular Identification of Three Walnuts (Juglans regia L.) Genotypes Grown in Egypt. test, 2022; 65(2): 651-658. doi: 10.21608/ejchem.2021.90859.4326

Fruit Physical, Chemical and Molecular Identification of Three Walnuts (Juglans regia L.) Genotypes Grown in Egypt

Egyptian Journal of Chemistry
Article 64, Volume 65, Issue 2, February 2022, Pages 651-658    PDF (328.79 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.90859.4326
Authors
Sami M AbouRayya1; Nabila El Badawy Kasem2; Eman Ibrahim* 3; Rasha G Salim4; Thanaa Sh.M. Mahmoud2
1horticultural crops technology NRC, Giza Egypt
2Horticulture crops Technology Department, Agricultural and Biological Division, National Research Centre
3Plant Biochemistry Department, National Research Center
4Microbial Genetic Department, National Research Centre (NRC), 33 EL Bohouth St., Dokki 12622, Cairo, Egypt.
Abstract
Three genotypes walnut (Juglans regia L.) were investigated during seasons 2019 & 2020 harvests from different selections of trees grown in El Gharbia governorate, Egypt. Physical properties, biochemical and molecular identification of fruit walnut types were examined. Physical properties showed that nut length ranged from 38 to 39.7 mm, nut width from 28.8 to 30.3 mm and nut weight from 11.10 to 12.61g. These genotypes are good quality according to the international walnut descriptor. The chemical analyses showed that total oil ranged from 45.84 to 61.99% while protein ranged from 8.88 to 14.12%. Total carbohydrate was in the range of 44.51-47.35% and the total phenol found between 137.97-147.54mg/g. The Palmitoleic, Oleic and Stearic acids were the main fatty acids in Type-1. Also, the polyunsaturated fatty acid especial linoleic acid was found predominant in Type-2 and Type-3. Stigmasterol (18.21%), Nonadecane (30.07%) and Octacosane (17.17%) were the major in Type-1. On the other hand Eicosane, b-Sitosterol, Pentacosane, and Nonadecane were major sterols in Type -2 while Octadecane was main in Type-3. Based on the DNA rbcL gene sequencing the three genotypes were identified as English walnuts and deposited in the Genbank database under accession numbers (MK002719 and MK002720).
Keywords
Walnut; Fruit quality; Nut traits,; Chemical composition; rbcl gene; Gen Bank
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