TARTOUGA, M., ELOUAR, I., ZEGHINA, I., MOKHTARI, M., GALI, L., RAPA, S., DE TOMMASI, N., MARZOCCO, S., BENSOUICI, C. (2022). The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract. test, 65(9), 109-118. doi: 10.21608/ejchem.2022.109501.5016
Maya Abir TARTOUGA; Ibtissem ELOUAR; Ibtissem ZEGHINA; Mohamed Badreddine MOKHTARI; Lynda GALI; Shara Francesca RAPA; Nunziatina DE TOMMASI; Stefania MARZOCCO; Chawki BENSOUICI. "The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract". test, 65, 9, 2022, 109-118. doi: 10.21608/ejchem.2022.109501.5016
TARTOUGA, M., ELOUAR, I., ZEGHINA, I., MOKHTARI, M., GALI, L., RAPA, S., DE TOMMASI, N., MARZOCCO, S., BENSOUICI, C. (2022). 'The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract', test, 65(9), pp. 109-118. doi: 10.21608/ejchem.2022.109501.5016
TARTOUGA, M., ELOUAR, I., ZEGHINA, I., MOKHTARI, M., GALI, L., RAPA, S., DE TOMMASI, N., MARZOCCO, S., BENSOUICI, C. The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract. test, 2022; 65(9): 109-118. doi: 10.21608/ejchem.2022.109501.5016

The evaluation of antioxidant and anti-inflammatory activities of Parmotrema hypotropa lichen extract

Egyptian Journal of Chemistry
Article 11, Volume 65, Issue 9, September 2022, Pages 109-118    PDF (386.14 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.109501.5016
Authors
Maya Abir TARTOUGA* 1; Ibtissem ELOUAR1; Ibtissem ZEGHINA1; Mohamed Badreddine MOKHTARI1; Lynda GALI2; Shara Francesca RAPA3; Nunziatina DE TOMMASI3; Stefania MARZOCCO3; Chawki BENSOUICI2
1Department of Animal Biology, Faculty of Natural and Life Sciences, University Frères Mentouri Constantine 1, route Ain el bey 25000, Constantine Algeria.
2National Center in Biotechnological Research (CRBT), 25 000 Constantine, Algeria.
3Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Italy.
Abstract
Lichens are used since antiquity for multiple therapeutic purposes, treating infections, fever, convulsions, and wounds. This study was designed to evaluate the antioxidant and anti-inflammatory effects of the lichen Parmotrema hypotropa (NYL.) Hale. The total phenolics and flavonoids content were determined in ethanol extract; the antioxidant activity was assessed by different chemical assays DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), β-carotene-linoleic acid, reducing power, and Cupric reducing antioxidant capacity (CUPRAC). The lichen extract was further tested in vitro, for its anti-inflammatory effect through the bovine serum albumin denaturation assay; also the inflammatory response was investigated on the LPS-stimulated human keratinocyte cell line (HaCaT) by measuring intercellular ROS (Reactive Oxygen Species) and TNF-α (Tumor necrosis factor-alpha) production; the amount of usnic acid in the extract was determined by High-Performance Liquid Chromatography (HPLC). The results showed that the tested extract contained high levels of total phenolics than flavonoids, and had an antioxidant effect; it prevented the protein denaturation in a dose-dependent manner, also reduced the intracellular ROS and TNF-α production by HaCaT cells. Furthermore, it did not affect the viability of HaCaT cells; the extract contained small amounts of usnic acid. Based on the different findings, P.hypotropa can constitute a potential source of bioactive compounds with antioxidant and anti-inflammatory effects.
Keywords
Lichen; Parmotrema hypotropa; antioxidant activity; anti-inflammatory effect; HaCaT cells; usnic acid
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