DNA Flow Cytometry Analysis Relation to Early and Late Clinical Stages in Oral Cancer Patients | ||
| Egyptian Journal of Oral and Maxillofacial Surgery | ||
| Article 3, Volume 12, Issue 3, July 2021, Pages 177-185 PDF (1000.19 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/omx.2021.59642.1108 | ||
| Authors | ||
| Ahmed Hussein* 1; Mohammed Khalil2; Ahmed Abdelrahman3; Asem Kamel4; Omar Soliman5; Omneya Ramadan6 | ||
| 1Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Assiut, Egypt | ||
| 2Department of Oral Pathology, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt | ||
| 3Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt | ||
| 4Department of Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt | ||
| 5Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Oral and Dental Medicine, South Valley University, Qena, Egypt | ||
| 6Department of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||
| Abstract | ||
| Introduction: Oral cancer is one of the critical global social health troubles. Lymph nodes metastasis of oral carcinomas have a significant prognosis impact. Authentic TNM staging of the neoplasms is essential to bear an assign board for disease control and prognostication. The model of grading histologically from only tissue biopsy has limitations. Abnormal DNA content has been correlated with malignant diseases. Flow cytometry can be a rapid method for quantitative test of DNA in solid tumors. Prediction the metastatic deposit in relation to the DNA flow cytometry inquiry is the point of importance. Aim of the study: This study was operated to matched between tumor DNA content to the clinical stages of oral squamous cell carcinoma patients; to better predict the tumor behavior and to guide treatment planning. Material and Method: A total of 40 fresh/frozen biopsies in different TNM clinical stages of oral cancer were divided into 2 groups. Group I; twenty lesions classified in stage I and stage II. Group II; twenty tumors classified as stage III and stage IV. All specimens utilized to DNA flow cytometric scanning in order to achieve the DNA ploidy and S-phase fraction. Results: The difference in aneuploidy state and S-phase fraction between Group I, and Group II was highly statistical significant in relation to their clinical stages on the basis of clinical information and biopsy results. Conclusions: DNA flow cytometric analysis of oral carcinomas excisions reported a significant predication to a late metastatic clinical stages of oral cancer lesions. | ||
| Keywords | ||
| DNA-Flow Cytometry; Aneuploidy; S-Phase Fraction; TNM System; Clinical Stages | ||
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