PATHOGENICITY OF ENTOMOPATHOGENIC BACTERIUM "XENORHABDUS NEMATOPHILA" AGAINST GALLERIA MELLONELLA L. AND SPODOPTERA LITTORALIS (BOISD.) | ||
| Egyptian Journal of Zoology | ||
| Article 6, Volume 63, Issue 63, June 2015, Pages 83-90 | ||
| Document Type: Original Research Papers | ||
| DOI: 10.12816/0014492 | ||
| Authors | ||
| M. A. Hussein1; G. M. Nouh2 | ||
| 1Pests and Plant Protection Department, National Research Centre, Giza, Egypt | ||
| 2Plant Protection Research Institute, Biological Control Department, Agriculture Research Center, Giza, Egypt | ||
| Abstract | ||
| Xenorhabdus nematophila (Enterobacteriaceae) is the symbiotic bacterium of an Egyptian strain of entomopathogenic nematode (EPN), Steinernema carpocapsae BA2 (Rhabditidae). In this study, the symbionts bacterium, Xenorhabdu snematophila BA2, was isolated from the haemolymph of the greater wax moth larvae, Galleria mellonella, infected with a native strain of S. carpocapsae (BA2). The efficiency of a fresh isolated bacterium was compared to 2-years old bacterium against the third-instar larvae of G. mellonella L. and cotton leaf worm Spodoptera littoralis (Boisd.) under laboratory conditions. The fresh bacteria caused an average of 51.5 and 54% mortalities to 3rd instar larvae of G. mellonella and S. littoralis, respectively. The 2-year old bacteria caused a respective of 48 and 52% mortalities. The mortality percentage increased as the concentration of entomopathogenic bacteria (EPB) increased. The LC50 values for X. nematophila reached 1.64x107 and 1.74x107 bacterial cells/ml for fresh and stored S. littoralis, respectively and 1.71x107 and 1.88x107 bacterial cells/ml for fresh and stored G. mellonella, respectively. | ||
| Keywords | ||
| Cotton leaf worm; Entomopathogenic bacterium; Honeycomb moth; pathogenicity; Septicemia; <i>Steinernema carpocapsae</i>; symbiosis | ||
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