Mohamed, N., El-Baghdady, K., El-Kholy, E., Fahmy, G. (2022). Prevalence of carbapenemase genes in extreme drug resistant Pseudomonas aeruginosa isolated from ICU in Egypt. test, 18(1), 39-51. doi: 10.21608/ajbs.2022.236833
Nermin M.K. Mohamed; Khaled Z. El-Baghdady; Eman El-Kholy; Gihan M. Fahmy. "Prevalence of carbapenemase genes in extreme drug resistant Pseudomonas aeruginosa isolated from ICU in Egypt". test, 18, 1, 2022, 39-51. doi: 10.21608/ajbs.2022.236833
Mohamed, N., El-Baghdady, K., El-Kholy, E., Fahmy, G. (2022). 'Prevalence of carbapenemase genes in extreme drug resistant Pseudomonas aeruginosa isolated from ICU in Egypt', test, 18(1), pp. 39-51. doi: 10.21608/ajbs.2022.236833
Mohamed, N., El-Baghdady, K., El-Kholy, E., Fahmy, G. Prevalence of carbapenemase genes in extreme drug resistant Pseudomonas aeruginosa isolated from ICU in Egypt. test, 2022; 18(1): 39-51. doi: 10.21608/ajbs.2022.236833

Prevalence of carbapenemase genes in extreme drug resistant Pseudomonas aeruginosa isolated from ICU in Egypt

African Journal of Biological Sciences
Article 4, Volume 18, Issue 1, June 2022, Pages 39-51    PDF (511.5 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2022.236833
Authors
Nermin M.K. Mohamed1; Khaled Z. El-Baghdady* 1; Eman El-Kholy2; Gihan M. Fahmy3
1Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
2Microbiology department, Ain Shams Specialized hospital, Cairo, Egypt
3Microbiology Department, Ain Shams Specialized Hospital, Cairo, Egypt
Abstract
The emergence of extreme drug resistant (XDR) Pseudomonas aeruginosa  represents a major problem in health care settings. This work aimed to study the prevalence of some OXA genes and biofilm formation among P. aeruginosa clinical isolates collected from Intensive Care Units (ICUs), Ain-Shams University, Cairo, Egypt. Among 70 Gram negative clinical isolates, 25 isolates were phenotypically identified as P. aeruginosa. Antibiotic sensitivity and minimum inhibitory concentration (MIC) for 18 different antibiotics (Ticarcillin, Ticarcillin /Clavulanic acid, Piperacillin, Piperacillin-Tazobactam, Ceftazidime, Cefepime, Meropenem, Amikacin, Gentamicin, Aztreonam, Imipenem, Meropenem, Tobramycin, Ciprofloxacin, Plefloxacin, Minocyclin, Rifampcin, Trimethothoprim/ Sulfamethoxazole and colistin) were carried out against P. aeruginosa isolates using VITEK 2.  All isolates were resistant to all antibiotics except colistin that only 12% were resistant. Real time polymerase chain reaction with specific primers was used to detect the presence of selected OXA genes. OXA group I and OXA group II were detected in 44 and 52%, respectively, while OXA group III, OXA51, OXA23, OXA 24 and OXA 58 were totally absent. Biofilm formation assay showed strong, weak and moderate biofilm formation in 44, 28 and 12% of the isolates, respectively, while 16% were non biofilm forming isolates.
Keywords
OXA; multidrug resistant; Pseudomonas aeruginosa and colistin
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