BIOCHEMICAL INTERACTION BETWEEN THE PLANT EXTRACTS AND THE ACTIVITIES OF A DIGESTIVE ENZYME, AMYLASE IN Spodoptera littoralis (80IS0.) | ||
| Journal of Plant Protection and Pathology | ||
| Article 5, Volume 29, Issue 5, May 2004, Pages 2865-2871 PDF (497.98 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jppp.2004.239719 | ||
| Author | ||
| L. A. Youssef, | ||
| Plant Protection Department, Faculty of Agriculture, Aln Shams University. | ||
| Abstract | ||
| Amylase enzyme was extracted from both anterior part of the foregut and posterior part of the hindgut of the fifth instar larvae of Egyptian cotton leaf worm, Spodoptera liftoralls (Boisd.).Then it was checked using starch and mercuric chloride as substrates. The present work shows that, activity of the enzyme depends on some important factors; incubatIon temperature, starvation and pH number .Amylase In the fifth instar larvae is acidic (optimum pH about 6) and the suitable Incubation temperature is 35 te. Starvation cause, an abrupt increase in the enzyme activity for the first four hours fonawed by a contlnious decrease of Us activity. Feeding larvae on castor bean fresh leaves dipped In extrads of either bestachia or dumb cane leaves in methanol and hexane, results in a significant inhibition in the activities of amylase enzyme In all treatments. | ||
| Keywords | ||
| Amylase; Bestachia and Dumb cane; Spectophotometer; Egyption cotton leaf worm | ||
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