Kishk,, W. (2003). EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.. test, 28(1), 115-128. doi: 10.21608/jappmu.2003.242175
W. H. Kishk,. "EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.". test, 28, 1, 2003, 115-128. doi: 10.21608/jappmu.2003.242175
Kishk,, W. (2003). 'EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.', test, 28(1), pp. 115-128. doi: 10.21608/jappmu.2003.242175
Kishk,, W. EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.. test, 2003; 28(1): 115-128. doi: 10.21608/jappmu.2003.242175

EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.

Journal of Animal and Poultry Production
Article 3, Volume 28, Issue 1, January 2003, Pages 115-128    PDF (3.71 M)
Document Type: Original Article
DOI: 10.21608/jappmu.2003.242175
Author
W. H. Kishk,
Animal Production Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Abstract
Two different methods of staining (Harris hematoxylin&Modified Bryan's
sperm stain) were used to explore sperm morphology changes after freezing for both
bull and buck sperm. Morphology sperm characters were concerned with state of the
following sperm organelles; head, med piece, acrosome, tail and cytoplasmic droplet.
Observations of abnormal sperm morphology were higher and differed significantly for
rabbit sperm than bull sperm after freezing. In addition both bull, buck sperm
morphology differed significantly before and after freezing. It couid be concluded that,
rabbit sperm is more sensitive for freezing process than bull sperm and it is still
needed for more appropriate freezing media to avoid freezing injuries at cellular and
sub cellular levels.
Keywords
Rabbit; Buck; Bull; Sperm; Staining; Acrosome; Frozen semen
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