Prevalence, Virulence and Antimicrobial Resistance of Salmonella Typhimurium in Retail Chicken Meat | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 13, Volume 27, Issue 1, June 2022, Pages 149-158 PDF (270.48 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2022.249303 | ||
| Abstract | ||
| Salmonella Typhimuriumwasisolated from food-borne outbreaks basically. The study pointed to reveal the overall prevalence of Salmonella in retail chicken meat, serotyping of Salmonella isolates to detect Salmonella Typhimuriumprevalence in retail chicken meat, antimicrobial susceptibility testing of recovered Salmonella Typhimuriumisolates, and stn, hilA, and pefA as virulence genes and blaTEM, blaCTX-M, blaSHV, blaCMY, blaDHA, qnrA, aadA1, tetA and sul1 as antimicrobial resistance genes. Out of 75 chicken meat samples, Two samples were positively identified as Salmonella with a total prevalence of 2.7 % (2/75). Salmonella isolates were serologically identified as Salmonella enterica serovar Typhimurium. All confirmed Salmonella isolates showed resistance to tetracycline (tetracycline), penicillins (ampicillin), aminoglycoside (gentamycin and streptomycin), sulphonamide (trimethoprim /sulfamethoxazole) and cephalosporins (cefuroxime). In comparison, 50% of the isolates were resistant to cephalosporins (cefotaxime and ceftazidime), nitrofuran (nitrofurantoin), and fluoroquinolones (levofloxacin and ciprofloxacin). Phenotypically, 50% of recovered isolates showed XRD (extensively drug resistance) to eight classes and 50% showed MRD (multidrug resistance). Concerning pefA, stn, and hilA as virulence genes, stn and hilA were detected in all tested Salmonella Typhimuriumby 100% (2/2), while pefA was not detected (0/2). Furthermore, all the following antimicrobial resistance genes were detected by 100 % as blaTEM, blaCTX-M and blaSHV, blaCMY, aadA1, tetA, and sul1. While blaDHA was not detected but qnrA was detected by 50%. In conclusion, raw retail chicken meat is heavily contaminated with XDR Salmonella Typhimurium. Moreover, important virulence factors in the isolates were also present, expanding the scope for public health concerns due to the potential for cross-contamination with other foods and the eating of undercooked poultry. | ||
| Keywords | ||
| Salmonella Typhimurium; serotyping; antimicrobial susceptibility testing; virulence genes; antimicrobial resistance genes | ||
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