Ismail, Y., Abbas, O., El Feky, H., Khamiss, A., Saied, S. (2020). Role of 16s rRNA Gene in Early Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascitic Patients. test, 29(1), 21-26. doi: 10.21608/ejmm.2020.249845
Yasser M. Ismail; Omaima M. Abbas; Hala M. El Feky; Afaf F. Khamiss; Sara A. Saied. "Role of 16s rRNA Gene in Early Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascitic Patients". test, 29, 1, 2020, 21-26. doi: 10.21608/ejmm.2020.249845
Ismail, Y., Abbas, O., El Feky, H., Khamiss, A., Saied, S. (2020). 'Role of 16s rRNA Gene in Early Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascitic Patients', test, 29(1), pp. 21-26. doi: 10.21608/ejmm.2020.249845
Ismail, Y., Abbas, O., El Feky, H., Khamiss, A., Saied, S. Role of 16s rRNA Gene in Early Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascitic Patients. test, 2020; 29(1): 21-26. doi: 10.21608/ejmm.2020.249845

Role of 16s rRNA Gene in Early Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascitic Patients

Egyptian Journal of Medical Microbiology
Volume 29, Issue 1, January 2020, Pages 21-26    PDF (372.28 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.249845
Authors
Yasser M. Ismail1; Omaima M. Abbas2; Hala M. El Feky3; Afaf F. Khamiss1; Sara A. Saied* 2
1Departments of Clinical Pathology, Faculty of Medicine, Benha University
2Clinical Pathology Department, National Liver Institute, Menoufia University
3Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Benha University
Abstract
Background: Ascites is a recognized problem in patients with decompensated liver cirrhosis. Spontaneous bacterial peritonitis (SBP) is a massive problem in patients who suffer from cirrhosis with ascites. Early bacterial detection allows great intervention to stop SBP. 16S ribosomal RNA (rRNA) is a universal gene used to detect different bacteria present within a sample. Objectives: This study aimed to evaluate the efficacy of broad range 16S rRNA gene polymerase chain reaction (PCR) in diagnosis of ascitic fluid (AF) infection. Methodology: Fifty cirrhotic ascitic patients were undergone to full history, clinical examination, laboratory tests including, AF specimens analysis for polymorph nuclear leucocytic (PMN) count, culture for bacteria and PCR- for DNA detection of bacteria. Results: Bacteria were separated from 21 (42%) of samples of ascitic fluid, and they were mainly Gram-negative bacteria. The sensitivities of culture for bacteria and PCR in diagnosing AF infection were 53% and 86% respectively, while the accuracies were 62% and 74% respectively relation with PMNL. Conclusions: Bacterial DNA in AF samples may be another method for diagnosis AF infection rather than bacterial culture and PMN count for early detection and treatment of AF infection.
Keywords
Spontaneous bacterial peritonitis; 16S ribosomal RNA; AF culture; Polymorph nuclear leucocytic count
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