Ghonaim, M., Labeeb, A., Eliwa, A., Salem, E. (2020). Molecular diagnosis and antifungal resistance and biofilm production profiles among Candida species isolated from immunocompromised patients at Menoufia University Hospitals. test, 29(3), 37-45. doi: 10.51429/EJMM29305
Mabrouk M. Ghonaim; Azza Z. Labeeb; Alyaa I. Eliwa; Eman H. Salem. "Molecular diagnosis and antifungal resistance and biofilm production profiles among Candida species isolated from immunocompromised patients at Menoufia University Hospitals". test, 29, 3, 2020, 37-45. doi: 10.51429/EJMM29305
Ghonaim, M., Labeeb, A., Eliwa, A., Salem, E. (2020). 'Molecular diagnosis and antifungal resistance and biofilm production profiles among Candida species isolated from immunocompromised patients at Menoufia University Hospitals', test, 29(3), pp. 37-45. doi: 10.51429/EJMM29305
Ghonaim, M., Labeeb, A., Eliwa, A., Salem, E. Molecular diagnosis and antifungal resistance and biofilm production profiles among Candida species isolated from immunocompromised patients at Menoufia University Hospitals. test, 2020; 29(3): 37-45. doi: 10.51429/EJMM29305

Molecular diagnosis and antifungal resistance and biofilm production profiles among Candida species isolated from immunocompromised patients at Menoufia University Hospitals

Egyptian Journal of Medical Microbiology
Volume 29, Issue 3, July 2020, Pages 37-45    PDF (554.22 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.51429/EJMM29305
Authors
Mabrouk M. Ghonaim; Azza Z. Labeeb; Alyaa I. Eliwa* ; Eman H. Salem
Department of Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt
Abstract
Background: Accurate and rapid identification of Candida species is necessary for proper diagnosis and treatment of candidiasis due to emergences of drug-resistant strains especially among immunocompromised patients. Objectives: Identification of Candida clinical isolates to the species level using different phenotypic and molecular methods. Biofilm-forming ability and antifungal resistance were also studied. Methodology: Sixty-nine Candida strains were isolated from 220 immunocompromised patients. Identification was performed using chromogenic Candida agar, VITEK 2 system and multiplex polymerase chain reaction (PCR). Biofilm formation was detected by the tube method and antifungal susceptibility was tested using the VITEK2 system. Results: The most common source of Candida isolates was from urine (33.3%) and ICUs (56.6%). VITEK 2 system detected 9 spp.: C. albicans (34.8%), C. tropicalis (21.7%), C. famata (8.7%), C. lusitaniae (7.2%), C. cruzi (7.2%), C. ciferri (5.8%), C. dubliniensis (5.8%), C. parapsilosis (5.8 %) and C. glabrata. Candida isolates showed high resistance to flucytocine (49.3%), and high sensitivity to fluconazole, micafungin, voriconazole and caspofungin (88.4%, 81.2% and 81.2 % respectively). Only 30.4% of all Candida isolates were biofilm producers. There was a positive relationship between antifungal resistance and biofilm formation among Candida isolates. Conclusion: C. albicans was the predominant species. Chromogenic Candida agar and VITEK 2 system were valuable tests compared to PCR in speciation of Candida isolates. Antifungal susceptibility was significantly related to biofilm production and its evaluation is important for proper treatment.
Keywords
Antifungal resistance; biofilm; Candida; chromogenic agar; molecular diagnosis; VITEK-2
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