Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. (2022). Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.. test, 3(3), 87-104. doi: 10.21608/ejmr.2022.253797
Sara Abd Eltawab Abd El fadeel; Hanan Dawood Yassa; Laila Ahmed Rashed; Gaber Hassan Abd El Fattah. "Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.". test, 3, 3, 2022, 87-104. doi: 10.21608/ejmr.2022.253797
Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. (2022). 'Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.', test, 3(3), pp. 87-104. doi: 10.21608/ejmr.2022.253797
Abd El fadeel, S., Yassa, H., Rashed, L., Abd El Fattah, G. Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.. test, 2022; 3(3): 87-104. doi: 10.21608/ejmr.2022.253797

Effect of Zinc Oxide Nanoparticles on The Spleen of Adult Male Albino Rat and The Possible Protective Role of Selenium: Microanatomical and Biochemical Study.

Egyptian Journal of Medical Research
Volume 3, Issue 3, July 2022, Pages 87-104    PDF (1.75 M)
Document Type: Original Article
DOI: 10.21608/ejmr.2022.253797
Authors
Sara Abd Eltawab Abd El fadeel1; Hanan Dawood Yassa1; Laila Ahmed Rashed2; Gaber Hassan Abd El Fattah1
1Anatomy and Embryology department,Faculty of Medicine, Beni-Suef University.
2Biochemistry department, Faculty of Medicine, Cairo University.
Abstract
Thirty five adult male albino rats were used in this study. They were divided into 7 groups: Group I (Control), Group II (low dose Zno NPs group) , Group III (medium dose Zno NPs group) , Group IV (high dose Zno NPs group), Group V (low dose Zno NPs-selenite group), Group VI (medium dose Zno NPs-selenite group), Group VII (high dose Zno NPs-selenite group): tissue of the spleen was examined histopathologically and immunohistochemical , Determination of blood cells count (number of white blood cells (WBCs), red blood corpuscles (RBCs) and platelets. Also tissue level of Hydrogen peroxide (H2O2) and paraoxonase-1(pon-1): for detection of oxidative stress estimated for all groups, PCR detection of apoptosis inducing factor (AIF), apoptotic protease activating factor-1 and caspases 3 and Zinc level in the spleen.
Results of the present study showed that, compared to controls: orally intake of Zinc oxide nanoparticles (Zno NPs) cause marked changes in spleen tissues as thickening of capsule, thickening of trabeculae , destruction of white pulp and red pulp expansion especially in groups receiving zinc oxide nanoparticles only and these changes markedly decreased on groups receiving selenium as protection
Keywords
Nanoparticles; ZnO NPs; selenium
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