MOSTAFA, R., ELAROUSY, M., SHALABY, M., ABDELAAL, A., MAHMOUD, S., BADAWI, M., ISMAIL, S., NAHNOUSH, R. (2022). CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE. test, 52(2), 231-238. doi: 10.21608/jesp.2022.257335
REHAM REFAAT MOSTAFA; MAHA HASSAN ELAROUSY; MAISA AHMED SHALABY; AMANY AHMED ABDELAAL; SOHEIR S. MAHMOUD; MANAL ABDELMAGED BADAWI; SHERIF MOHAMED ISMAIL; REHAM K. NAHNOUSH. "CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE". test, 52, 2, 2022, 231-238. doi: 10.21608/jesp.2022.257335
MOSTAFA, R., ELAROUSY, M., SHALABY, M., ABDELAAL, A., MAHMOUD, S., BADAWI, M., ISMAIL, S., NAHNOUSH, R. (2022). 'CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE', test, 52(2), pp. 231-238. doi: 10.21608/jesp.2022.257335
MOSTAFA, R., ELAROUSY, M., SHALABY, M., ABDELAAL, A., MAHMOUD, S., BADAWI, M., ISMAIL, S., NAHNOUSH, R. CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE. test, 2022; 52(2): 231-238. doi: 10.21608/jesp.2022.257335

CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE

Journal of the Egyptian Society of Parasitology
Volume 52, Issue 2, August 2022, Pages 231-238    PDF (1.33 M)
DOI: 10.21608/jesp.2022.257335
Authors
REHAM REFAAT MOSTAFA1; MAHA HASSAN ELAROUSY1; MAISA AHMED SHALABY2; AMANY AHMED ABDELAAL3; SOHEIR S. MAHMOUD2; MANAL ABDELMAGED BADAWI4; SHERIF MOHAMED ISMAIL5; REHAM K. NAHNOUSH3
1Department of Medical Parasitology, Faculty of Medicine, Cairo University
2Department of Parasitology, Theodor Bilharz Research Institute (TBRI)
3Department of Medical Parasitology, Faculty of Medicine
4Department of Pathology , National Research Institute
5Department of Pathology, Faculty of Medicine, Cairo University
Abstract
The most serious form of chronic schistosomiasis is the life-threatening hepatosplenic disease,
accompanied by severe periportal fibrosis, a permanent condition once established. Reversion of
such wound healing process “fibrolysis” is possible if a balance between hepatic cellular regeneration
and the process of scar formation is ensued. In the current work, clove oil alone and combined
with PZQ were used to investigate their anti-fibrotic effect on hepatic fibrosis resulted from
acute and chronic schistosomiasis mansoni in mice. To determine the state of hepatic fibroblasts,
local expression of TGF-β1, a chief profibrogenic molecule was quantified using digital realtime
image analysis and compared with the conventional parasitological and histopathological
analysis. PZQ monotherapy caused a significant reduction in ova count, granuloma number and
size. Local expression of TGF-β1 gave better data as compared to acute and chronic infected
treated mice (11.91±2.53- 20.34±3.05 vs. 7.51±2.11-11.23±2.23) with clove alone treatment &
4.95±1.95-7.51±1.92 in combined therapy (p=0.0000)), indicated low potential in achieving liver
tissue repair. Significant drop in TGF-β1 expression with clove oil treatment, especially when
combined with PZQ, indicated anti-fibrotic potentiality and good impact on liver cells proceeded
towards regeneration supported by such drop. A significant positive correlation between mean
TGF-β1 values and mean granuloma parameters in number and size of different infected groups
(R² = 0.680 & 0.988, respectively in acute infection, 0.363 & 0.505 respectively, in chronic phase
of infection, P value <0.05).
Keywords
Mice; Schistosoma mansoni; Clove oil; Praziquantel; Hepatic fibrosis; TGF-β1
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